Morphology and MMP-9, AR and IGFR-1 responses of the seminal vesicle in TRAMP mice model

Autor(es)

Sanches Dal Pozzo, Caroline Fernanda ^[1] ; Kido, Larissa Akemi ^[1] ; Montico, Fabio ^[1] ; Goncalves, Mariana Piccoli ^[1] ; Alves Cagnon, Valeria Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Struct & Cellular Biol Postgrad Program, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TISSUE & CELL; v. 48, n. 3, p. 217-223, JUN 2016.
• Citações Web of Science: 2

Resumo

Seminal vesicles are important hormone-dependent accessory sex glands. Transgenic adenocarcinoma of the mouse prostate (TRAMP) model has been used to evaluate malignant diseases in the prostate and in other sexual glands. The aim of this study was to characterize structural and molecular features of the seminal vesicle in different life periods of the TRAMP mice. Groups: Control Group (5 FVB/12 week old mice), TRAMP 12 and 22 Groups (10 TRAMP 12 and 22 week old mice, respectively). Seminal vesicles were evaluated by morphological and immunohistochemical parameters; androgenic receptor (AR), Insulin-like growth factor 1 (IGFR-1) and metalloproteinase 9 (MMP-9). The TRAMP mice showed frequent epithelial proliferation, including cellular stromal invasion, especially in the TRAMP 22 group. Intense AR reactivity was seen in both stroma and epithelial regions in the TRAMP 22 group. Intense IGFR1 and MMP-9 stromal immunolabeling was identified in both TRAMP groups. Thus, there were structural and molecular changes in the seminal vesicle in TRAMP mice, compromising not only the structure but also the stromal signaling, damaging thus the function and leading to glandular lesions. TRAMP mice could be indicated as a good model to study alterations of the seminal vesicle in association to prostate cancer. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/08632-6 - Caracterização estrutural e molecular da vesícula seminal de camundongos transgênicos para adenocarcinoma de próstata (TRAMP) e correlação com o microambiente glandular de camundongos senis (FVB)
• Beneficiário: Caroline Fernanda Sanches Dal Pozzo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica