Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Beta-2-microglobulin (B2M) expression in the urinary sediment correlates with clinical markers of kidney disease in patients with type 1 diabetes

Texto completo
Autor(es):
Monteiro, Maria Beatriz [1] ; Thieme, Karina [1] ; Santos-Bezerra, Daniele Pereira [1] ; Queiroz, Marcia Silva [2] ; Woronik, Viktoria [3] ; Passarelli, Marisa [4] ; Machado, Ubiratan Fabres [5] ; Giannella-Neto, Daniel [6] ; Oliveira-Souza, Maria [7] ; Correa-Giannella, Maria Lucia [1, 8]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Lab Cellular & Mol Endocrinol LIM 25, Au Dr Arnaldo 455 4305, BR-01246903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Hosp Clin, Div Endocrine, Sch Med, Av Dr Eneas de Carualho Aguiar 155, BR-05403000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Lab Renal Pathophysiol LIM 16, Av Dr Arnaldo 455 3345, BR-01246903 Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Lipids Lab LIM 10, Av Dr Arnaldo 455 3305, BR-01246903 Sao Paulo - Brazil
[5] Inst Biomed Sci, Lab Metab & Endocrinol, Dept Physiol & Biophys, Av Prof Lineu Prestes 1524 126, BR-05508000 Sao Paulo - Brazil
[6] Univ Nove Julho UNINOVE, Programa Posgrad Med, R Vergueiro 235-249, BR-01504001 Sao Paulo - Brazil
[7] Inst Biomed Sci, Dept Physiol & Biophys, Lab Renal Physiol, Av Prof Lineu Prestes 1524 227, BR-05508000 Sao Paulo - Brazil
[8] Univ Sao Paulo, Sch Med, Cell & Mol Therapy Ctr NUCEL, Lab Cellular & Mol Endocrinol LIM 25, BR-05360120 Sao Paulo - Brazil
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: METABOLISM-CLINICAL AND EXPERIMENTAL; v. 65, n. 6, p. 816-824, JUN 2016.
Citações Web of Science: 8
Resumo

Purpose. After observing variation in the expression of the housekeeping gene B2M in cells of the urinary sediment during a study of candidate genes potentially involved in diabetic kidney disease (DKD), we hypothesized that B2M mRNA expression in the urinary sediment could reflect the presence of DKD. Methods. qPCR was used to quantify B2M mRNA expression in cells of the urinary sediment of 51 type 1 diabetes (T1D) patients (61% women, 33.5 {[}27.0-39.7] years old, with diabetes duration of 21.0 {[}15.0-28.0] years and HbA1c of 8.2% {[}7.3-8.9]; median {[}interquartile interval]) sorted according to the diabetic nephropathy (DN) stages; 8 focal segmental glomerulosclerosis (FSGS) patients and 10 healthy controls. B2M mRNA expression was also evaluated in human embryonic kidney epithelium-like (HEK-293) cells exposed to 25 mM glucose and to albumin in order to mimic, respectively, a diabetic and a proteinuric milieu. Results. No differences were found in B2M mRNA expression among healthy controls, FSGS and T1D patients. Nonetheless B2M mRNA expression was higher in the group composed by T1D patients with incipient or overt DN combined with FSGS patients versus T1D patients without DN combined with healthy controls (P = 0.0007). B2M mRNA expression was higher in T1D patients with incipient or overt DN versus without DN (P = 0.03). B2M mRNA expression positively correlated with albuminuria in the overall T1D population (r = 0.43; P = 0.01) and negatively correlated with estimated glomerular filtration rate in male T1D patients (r = - 0.57; P = 0.01). Increased B2M expression was observed in HEK-293 cells exposed to 25 mM glucose and to albumin. Conclusions. B2M mRNA expression in cells of the urinary sediment is higher in T1D patients with DKD and in patients with FSGS in comparison to healthy subjects, maybe reflecting a tubulointerstitial injury promoted by albumin. Given the proinflammatory nature of B2M, we suggest that this protein contributes to diabetic (and possibly, to non-diabetic) tubulopathy. (C) 2016 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 12/04831-1 - Novos moduladores do controle glicêmico e do desenvolvimento de complicações crônicas no Diabetes mellitus: perspectivas preventivas e terapêuticas
Beneficiário:Ubiratan Fabres Machado
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 12/05583-1 - Expressão do RNA mensageiro dos genes TXNIP, SCL19A2 e SCL19A3 no sedimento urinário de portadores de Diabetes mellitus tipo 1 com e sem nefropatia diabética
Beneficiário:Maria Lucia Cardillo Corrêa Giannella
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/19569-3 - Efeitos da endotelina 3 e da leptina na morfologia e função renal in vivo: participação do sistema renina-angiotensina intrarrenal
Beneficiário:Maria Oliveira de Souza
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/02003-4 - Expressão do RNA mensageiro dos genes TXNIP, SCL19A2 e SCL19A3 no sedimento urinário de portadores de diabetes mellitus tipo 1 com e sem nefropatia diabética
Beneficiário:Maria Beatriz Camargo Monteiro Caillaud
Linha de fomento: Bolsas no Brasil - Doutorado