Euphorbia tirucalli modulates gene expression in larynx squamous cell carcinoma

Autor(es)

Franco-Salla, Gabriela Bueno ^[1] ; Prates, Janesly ^[1] ; Cardin, Laila Toniol ^[1] ; Dinarte dos Santos, Anemari Ramos ^[2] ; da Silva, Jr., Wilson Araujo ^[2] ; da Cunha, Bianca Rodrigues ^[3] ; Tajara, Eloiza Helena ^[3] ; Oliani, Sonia Maria ^[1] ; Rodrigues-Lisoni, Flavia Cristina ^[4]

Afiliação do(s) autor(es)

^[1] UNESP, Inst Biosci Letters & Sci IBILCE, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Sao Paulo, FCFRP USP, Fac Med Ribeirao Preto, Dept Clin Med, Fdn Blood Ctr Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Fac Med Sao Jose do Rio Preto FAMERP, Dept Mol Biol, Sao Jose Do Rio Preto, SP - Brazil

^[4] FEIS UNESP, Fac Engn Ilha Solteira, Dept Biol & Anim Sci, Av Brasil 56, BR-15385000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE; v. 16, MAY 21 2016.
• Citações Web of Science: 4

Resumo

Background: Some plants had been used in the treatment of cancer and one of these has attracted scientific interest, the Euphorbia tirucalli (E. tirucalli), used in the treatment of asthma, ulcers, warts has active components with activities scientifically proven as antimutagenic, anti-inflammatory and anticancer. Methods: We evaluate the influence of the antitumoral fraction of the E. tirucalli latex in the larynx squamous cell carcinoma (Hep-2), on the morphology, cell proliferation and gene expression. The Hep-2 cells were cultivated in complete medium (MEM 10 %) and treated with E. tirucalli latex for 1, 3, 5 and 7 days. After statistically analyzing the proliferation of the tested cells, the cells were cultivated again for RNA extraction and the Rapid Subtractive Hybridization (RaSH) technique was used to identify genes with altered expression. The genes found using the RaSH technique were analyzed by Gene Ontology (GO) using Ingenuity Systems. Results: The five genes found to have differential expression were validated by real-time quantitative PCR. Though treatment with E. tirucalli latex did not change the cell morphology in comparison to control samples, but the cell growth was significantly decreased. The RaSH showed change in the expression of some genes, including ANXA1, TCEA1, NGFRAP1, ITPR1 and CD55, which are associated with inflammatory response, transcriptional regulation, apoptosis, calcium ion transport regulation and complement system, respectively. The E. tirucalli latex treatment down-regulated ITPR1 and up-regulated ANXA1 and CD55 genes, and was validated by real-time quantitative PCR. Conclusions: The data indicate the involvement of E. tirucalli latex in the altered expression of genes involved in tumorigenic processes, which could potentially be applied as a therapeutic indicator of larynx cancer. (AU)

• Processo FAPESP: 13/24083-2 - Investigação da modulação da proteína anti-inflamatória anexina A1 em diferentes células de câncer de colo de útero
• Beneficiário: Flávia Cristina Rodrigues Lisoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/07853-6 - Análise da expressão gênica em resposta ao tratamento com Euphorbia tirucalli (Aveloz) em carcinoma epidermóide de laringe
• Beneficiário: Gabriela Bueno Franco
• Modalidade de apoio: Bolsas no Brasil - Mestrado