| Texto completo | |
| Autor(es): Mostrar menos - |
Barros, Michele S.
[1]
;
Gomes, Eliane
[1]
;
Gueroni, Daniele I.
[1]
;
Ramos, Anderson D.
[1]
;
Mirotti, Luciana
[1]
;
Florsheim, Esther
[1]
;
Bizzarro, Bruna
[1]
;
Lino, Ciro N. R.
[1, 2]
;
Maciel, Ceres
[1]
;
Lino-Dos-Santos-Franco, Adriana
[3]
;
Tavares-de-Lima, Wothan
[4]
;
Capurro, Margareth L.
[5, 6]
;
Russo, Momtchilo
[1]
;
Sa-Nunes, Anderson
[1, 6]
Número total de Autores: 14
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo, SP - Brazil
[2] Hannover Med Sch, Inst Immunol, Hannover - Germany
[3] Univ Nove de Julho UNINOVE, Grad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo, SP - Brazil
[6] Natl Council Sci & Technol Dev INCT EM CNPq, Natl Inst Sci & Technol Mol Entomol, Rio De Janeiro, RJ - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 11, n. 5 MAY 20 2016. |
| Citações Web of Science: | 1 |
| Resumo | |
Classical studies have shown that Aedes aegypti salivary secretion is responsible for the sensitization to mosquito bites and many of the components present in saliva are immunogenic and capable of inducing an intense immune response. Therefore, we have characterized a murine model of adjuvant-free systemic allergy induced by natural exposure to mosquito bites. BALB/c mice were sensitized by exposure to A. aegypti mosquito bites and intranasally challenged with phosphate-buffered saline only or the mosquito's salivary gland extract (SGE). Blood, bronchoalveolar lavage (BAL) and lung were collected and evaluated for cellularity, histopathological analyses, cytokines and antibody determination. Respiratory pattern was analyzed by Penh measurements and tracheal segments were obtained to study in vitro reactivity to methacholine. BAL recovered from sensitized mice following challenge with SGE showed an increased number of eosinophils and Th2 cytokines such as IL-4, IL-5 and IL-13. Peribronchoalveolar eosinophil infiltration, mucus and collagen were also observed in lung parenchyma of sensitized mice, suggesting the development of a typical Th2 response. However, the antibody profile in serum of these mice evidenced a mixed-type response with presence of both, IgG1/IgE (Th2-related) and IgG2a (Th1-related) isotypes. In addition, changes in breathing pattern and tracheal reactivity to methacholine were not found. Taken together, our results show that A. aegypti bites trigger an atypical allergic reaction, with some classical cellular and soluble Th2 components in the lung, but also systemic Th1 and Th2 antibody isotypes and no change in either the respiratory pattern or the trachea responsiveness to agonist. (AU) | |
| Processo FAPESP: | 11/06626-3 - Modelos experimentais in vitro e in vivo e citometria de fluxo. |
| Beneficiário: | Daniele Intini Gueroni |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 11/15569-3 - Caracterização da atividade biológica da serpina salivar AET-7393 de Aedes aegypti |
| Beneficiário: | Ciro Novaes Rosa Lino |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 10/18216-1 - Papel da imunidade do hospedeiro no fitness biológico do mosquito vetor Aedes aegypti |
| Beneficiário: | Ceres Maciel de Miranda |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 09/09892-6 - Imunoma funcional da saliva de Aedes aegypti |
| Beneficiário: | Anderson de Sá Nunes |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 09/07208-0 - Modulação da inflamação pulmonar alérgica por adjuvantes ou tolerância imunológica |
| Beneficiário: | Momtchilo Russo |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 09/12247-5 - Efeito da saliva de Aedes aegypti sobre a diferenciação, maturação e função de células dendríticas |
| Beneficiário: | Bruna Bizzarro |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 11/22669-4 - Imunomodulação da encefalomielite autoimune experimental pelo extrato da glândula salivar de Aedes aegypti |
| Beneficiário: | Anderson Daniel Ramos |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |