beta 2-Adrenoceptor is involved in connective tissue remodeling in regenerating muscles by decreasing the activity of MMP-9

Autor(es)

Silva, Meiricris T. ^[1] ; Nascimento, Tabata L. ^[1] ; Pereira, Marcelo G. ^[1] ; Siqueira, Adriane S. ^[2] ; Brum, Patricia C. ^[3] ; Jaeger, Ruy G. ^[2] ; Miyabara, Elen H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Ave Prof Lineu Prestes 2415, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Phys Educ & Sport, Dept Biodynam, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell and Tissue Research; v. 365, n. 1, p. 173-186, JUL 2016.
• Citações Web of Science: 0

Resumo

We investigated the role of beta 2-adrenoceptors in the connective tissue remodeling of regenerating muscles from beta 2-adrenoceptor knockout (beta 2KO) mice. Tibialis anterior muscles from beta 2KO mice were cryolesioned and analyzed after 3, 10, and 21 days. Regenerating muscles from beta 2KO mice showed a significant increase in the area density of the connective tissue and in the amount of collagen at 10 days compared with wild-type (WT) mice. A greater increase occurred in the expression levels of collagen I, III, and IV in regenerating muscles from beta 2KO mice evaluated at 10 days compared with WT mice; this increase continued at 21 days, except for collagen III. Matrix metalloproteinase (MMP-2) activity increased to a similar extent in regenerating muscles from both beta 2KO and WT mice at 3 and 10 days. This was also the case for MMP-9 activity in regenerating muscles from both beta 2KO and WT mice at 3 days; however, at 10 days post-cryolesion, this activity returned to baseline levels only in WT mice. MMP-3 activity was unaltered in regenerating muscles at 10 days. mRNA levels of tumor necrosis factor-alpha increased in regenerating muscles from WT and beta 2KO mice at 3 days and, at 10 days post-cryolesion, returned to baseline only in WT mice. mRNA levels of interleukin-6 increased in muscles from WT mice at 3 days post-cryolesion and returned to baseline at 10 days post-cryolesion but were unchanged in beta 2KO mice. Our results suggest that the beta 2-adrenoceptor contributes to collagen remodeling during muscle regeneration by decreasing MMP-9 activity. (AU)

• Processo FAPESP: 14/13874-1 - Regeneração muscular esquelética de camundongos com Síndrome de Marfan: estudo do potencial regenerativo das células satélites
• Beneficiário: Meiricris Tomaz da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/23391-8 - Resposta regenerativa da musculatura esquelética de camundongos com síndrome de Marfan
• Beneficiário: Elen Haruka Miyabara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/52520-0 - Efeito da suplementacao com leucina no processo de regeneracao muscular esqueletica.
• Beneficiário: Marcelo Gomes Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/04783-0 - Influência das proteínas de choque térmico na resposta regenerativa de músculos esqueléticos de camundongos idosos
• Beneficiário: Tábata Leal Nascimento
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/21238-2 - Plasticidade do músculo esquelético após lesão: papel do receptor beta2-adrenérgico
• Beneficiário: Elen Haruka Miyabara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/54240-7 - Papel do adrenocpetor beta 2 na regeneracao muscular esqueletica
• Beneficiário: Meiricris Tomaz da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado