The citrus flavonone naringenin reduces lipopolysaccharide-induced inflammatory pain and leukocyte recruitment by inhibiting NF-kappa B activation

Autor(es):Mostrar menos -

Pinho-Ribeiro, Felipe A. ^[1] ; Zarpelon, Ana C. ^[1] ; Mizokami, Sandra S. ^[1] ; Borghi, Sergio M. ^[1] ; Bordignon, Juliano ^[2] ; Silva, Rangel L. ^[3] ; Cunha, Thiago M. ^[3] ; Alves-Filho, Jose C. ^[3] ; Cunha, Fernando Q. ^[3] ; Casagrande, Rubia ^[4] ; Verri, Jr., Waldiceu A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Londrina, Ctr Ciencias Biol, Dept Ciencias Patol, Rod Celso Garcia Cid, Km 380, PR445, BR-PR445860 Londrina, Parana - Brazil

^[2] Fiocruz MS, Carlos Chagas Inst, Rua Prof Algacyr Munhoz Mader 3775, BR-81350010 Curitiba, Parana - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ave Bandeirantes, BR-14050490 Ribeirao Preto, SP - Brazil

^[4] Univ Estadual Londrina, Ctr Ciencias Saude, Univ Hosp, Dept Ciencias Farmaceut, Ave Robert Koch 60, BR-86038350 Londrina, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 33, p. 8-14, JUL 2016.
• Citações Web of Science: 35

Resumo

Lipopolysaccharide (LPS) is the major structural component of Gram-negative bacteria cell wall and a highly pro-inflammatory toxin. Naringenin is found in Citrus fruits and exhibits antioxidant and anti-inflammatory properties through inhibition of NF-kappa B activation but its effects in LPS-induced inflammatory pain and leukocyte recruitment were not investigated yet. We investigated the effects of naringenin in mechanical hyperalgesia, thermal hyperalgesia and leukocyte recruitment induced by intraplantar injection of LPS in mice. We found that naringenin reduced hyperalgesia to mechanical and thermal stimuli, myeloperoxidase (MPO, a neutrophil and macrophage marker) and N-acetyl-beta-D-glucosaminidase (NAG, a macrophage marker) activities, oxidative stress and cytokine (TNF-alpha, IL-1 beta, IL-6, and IL-12) production in the paw skin. In the peritoneal cavity, naringenin reduced neutrophil and mononuclear cell recruitment, and abrogated MPO and NAG activity, cytokine and superoxide anion production, and lipid peroxidation. In vitro, pre-treatment with naringenin inhibited superoxide anion and cytokine (TNF-alpha, IL-1 beta, IL-6, and IL-12) production by LPS-stimulated RAW 264.7 macrophages. Finally, we demonstrated that naringenin inhibited NF-kappa B activation in vitro and in vivo. Therefore, naringenin is a promising compound to treat LPS-induced inflammatory pain and leukocyte recruitment. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 11/19670-0 - Mecanismos envolvidos na fisiopatologia da artrite reumatóide, dor e sepse
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Temático