Prevalence of human papillomavirus types and variants and p16(INK4a) expression in head and neck squamous cells carcinomas in Sao Paulo, Brazil

Autor(es):Mostrar menos -

Betiol, Julio C. ^{[1, 2]} ; Sichero, Laura ^{[1, 2, 3]} ; de Olival Costa, Henrique O. ^[4] ; de Matos, Leandro L. ^[5] ; Andreoli, Maria A. ^[6] ; Ferreira, Silvaneide ^{[1, 2]} ; Faraj, Sheila F. ^[7] ; de Mello, Evandro S. ^[7] ; Sobrinho, Joao S. ^{[1, 2]} ; Brandao, Lenine G. ^[5] ; Cernea, Claudio R. ^[5] ; Kulcsar, Marco A. ^[5] ; Pinto, Fabio R. ^[5] ; Goncalves, Antonio J. ^[8] ; Menezes, Marcelo B. ^[8] ; Silva, Leonardo ^[4] ; Rossi, Lia M. ^[4] ; Lima Nunes, Rafaella A. ^[6] ; Termini, Lara ^[6] ; Villa, Luisa L. ^{[9, 1, 2, 6]}

Afiliação do(s) autor(es)

^[1] ICESP, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Mol Biol Lab, Hosp Clin, Ctr Translat Res Oncol, Inst Canc Estado Sao Paulo, Sao Paulo - Brazil

^[3] Ctr Translat Oncol ICESP, Av Dr Arnaldo 251, 8 Andar, BR-01246000 Sao Paulo, SP - Brazil

^[4] Sch Med FCMSCSP, Dept Otolaringol, Santa Casa Sao Paulo, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Med, Dept Head & Neck Surg, Sao Paulo - Brazil

^[6] Sch Med FCMSCSP, HPV Inst, Santa Casa Sao Paulo, Sao Paulo - Brazil

^[7] Canc Inst Sao Paulo ICESP, Dept Pathol, Sao Paulo - Brazil

^[8] Sch Med FCMSCSP, Dept Surg, Santa Casa Sao Paulo, Sao Paulo - Brazil

^[9] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFECTIOUS AGENTS AND CANCER; v. 11, MAY 4 2016.
• Citações Web of Science: 5

Resumo

Background: Human papillomavirus (HPV) prevalence in head and neck squamous cell carcinomas (HNSCC) diverges geographically. The reliability of using p16(INK4a) expression as a marker of viral infection is controversial in HNSCC. We evaluated HPV types and HPV-16 variants prevalence, and p16(INK4a) expression in HNSCC specimens provided by two different Institutions in Sao Paulo. Methods: HPV DNA from formalin-fixed specimens was accessed by Inno-LiPA, HPV-16 variants by PCR-sequencing, and p16(INK4a) protein levels by immunohistochemistry. Results: Overall, HPV DNA was detected among 19.4 % of the specimens (36/186). Viral prevalence was higher in the oral cavity (25.0 %, 23/92) then in other anatomical sites (oropharynx 14,3 %, larynx 13.7 %) when samples from both Institutions were analyzed together. HPV prevalence was also higher in the oral cavity when samples from both Institutions were analyzed separately. HPV-16 was the most prevalent type identified in 69.5 % of the HPV positive smaples and specimens were assigned into Asian-American (57.2 %) or European (42.8 %) phylogenetic branches. High expression of p16(INK4a) was more common among HPV positive tumors. Conclusion: Our results support a role for HPV-16 in a subset of HNSCC. (AU)

• Processo FAPESP: 12/01513-9 - Diversidade de HPV e Risco de Câncer
• Beneficiário: Julio Cesar Betiol
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/57889-1 - Instituto de Ciência e Tecnologia para o Estudo das Doenças Associadas ao Papilomavírus
• Beneficiário: Luisa Lina Villa
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/09616-9 - Diversidade de HPV e risco de câncer
• Beneficiário: Luisa Lina Villa
• Modalidade de apoio: Auxílio à Pesquisa - Regular