Regulatory and junctional proteins of the blood-testis barrier in human Sertoli cells are modified by monobutyl phthalate (MBP) and bisphenol A (BPA) exposure

Autor(es)

Aquino Goncalves de Freitas, Andre Teves ^[1] ; Ribeiro, Mariana Antunes ^[1] ; Pinho, Cristiane Figueiredo ^[1] ; Peixoto, Andre Rebelo ^[1] ; Domeniconi, Raquel Fantin ^[2] ; Scarano, Wellerson R. ^[3]

Afiliação do(s) autor(es)

^[1] UNESP, Inst Biosci, Grad Program Gen & Appl Biol, Botucatu, SP - Brazil

^[2] UNESP, Inst Biosci, Dept Anat, Botucatu, SP - Brazil

^[3] UNESP, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY IN VITRO; v. 34, p. 1-7, AUG 2016.
• Citações Web of Science: 9

Resumo

The blood-testis barrier (BTB) is responsible for providing a protected environment and coordinating the spermatogenesis. Endocrine disruptors (EDs) might lead to infertility, interfering in the BTB structure and modulation. This study aimed to correlate the actions of two EDs, monobutyl phthalate (MBP) and bisphenol A (BPA) in different periods of exposure, in a low toxicity dose to the human Sertoli cells{''}(HSeC) and its effects on the proteins of the BTB and regulatory proteins involved in its modulation. HSeC cells were exposed to MBP (10 mu M) and BPA (20 mu M) for 6 and 48 h. Western Blot assay indicated that MBP was able to reduce the expression of occludin, ZO-1, N-cadherin and Androgen Receptor (AR), while BPA leads to a reduction of occludin, ZO-1, beta-catenin and AR. TGF-beta 2 and F-actin were not modified. Phalloidin and Hematoxylin and Eosin assay revealed phenotically disruption in Sertoli cells adhesion, without changes in F-actin expression or localization. Our data suggested both EDs present potential for disrupting the structure and maintenance of the human BTB by AR dependent pathway. (C) 2016 Published by Elsevier Ltd. (AU)

• Processo FAPESP: 12/00253-3 - Análise do padrão de metilação das regiões controladoras de imprinting dos genes H19 e IGF2 e da resposta androgênica em células de Sertoli humanas expostas ao TCDD (2,3,7,8-tetraclorodibenzeno-p-dioxina)
• Beneficiário: Wellerson Rodrigo Scarano
• Modalidade de apoio: Auxílio à Pesquisa - Regular