In vivo and in vitro phagocytosis of Leishmania (Leishmania) amazonensis promastigotes by B-1 cells

Autor(es)

Geraldo, M. M. ; Costa, C. R. ; Barbosa, F. M. C. ; Vivanco, B. C. ; Gonzaga, W. F. K. M. ; Novaes e Brito, R. R. ; Popi, A. F. ; Lopes, J. D. ; Xander, P.

• Tipo de documento: Artigo Científico
• Fonte: PARASITE IMMUNOLOGY; v. 38, n. 6, p. 365-376, JUN 2016.
• Citações Web of Science: 6

Resumo

Leishmaniasis is caused by Leishmania parasites that infect several cell types. The promastigote stage of Leishmania is internalized by phagocytic cells and transformed into the obligate intracellular amastigote form. B-1 cells are a sub-population of B cells that are able to differentiate in vitro and in vivo into mononuclear phagocyte-like cells with phagocytic properties. B-1 cells use several receptors for phagocytosis, such as the mannose receptor and third complement receptor. Leishmania binds to the same receptors on macrophages. In this study, we demonstrated that phagocytes derived from B-1 cells (B-1 CDP) were able to internalize promastigotes of L. (L.) amazonensis in vitro. The internalized promastigotes differentiated into amastigotes. Our results showed that the phagocytic index was higher in B-1 CDP compared to peritoneal macrophages and bone marrow-derived macrophages. The in vivo phagocytic ability of B-1 cells was also demonstrated. Parasites were detected inside purified B-1 cells after intraperitoneal infection with L. (L.) amazonensis promastigotes. Intraperitoneal stimulation with the parasites led to an increase in both IL-10 and TNF-alpha. These results highlight the importance of studying B-1 CDP cells as phagocytic cells that can participate and contribute to immunity to parasites. (AU)

• Processo FAPESP: 12/11837-6 - Avaliação da participação de linfócitos B-1 na leishmaniose experimental
• Beneficiário: Patricia Xander Batista
• Modalidade de apoio: Auxílio à Pesquisa - Regular