Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genotyping and differential expression analysis of inflammasome genes in sporadic malignant melanoma reveal novel contribution of CARD8, IL1B and IL18 in melanoma susceptibility and progression

Texto completo
Autor(es):
da Silva, Wanessa Cardoso ; Oshiro, Telma Miyuki ; de Sa, Daniel Coelho ; Franco, Dilcilea D. G. S. ; Neto, Cyro Festa ; Pontillo, Alessandra
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: CANCER GENETICS; v. 209, n. 10, p. 474-480, OCT 2016.
Citações Web of Science: 8
Resumo

Sporadic melanoma malignancy is correlated with constitutive secretion of IL-1 beta in transformed melanocytes suggesting the involvement of inflammasome in melanoma. Common variants in inflammasome genes are known to affect IL-1 beta expression. To investigate the contribution of inflammasome genetics in melanoma development and progression and to identify a potential prognostic marker, the distribution of selected inflammasome SNPs was analysed in a Brazilian case/control cohort of sporadic malignant melanoma (SMM) and then the expression of inflammasome components was evaluated in melanoma biopsies. Allele and gene-specific Taqman assays were implied for genotyping of case/control DNA samples and for relative expression analysis in skin biopsies respectively. CARD8 rs6509365 was found to be significantly more common in healthy volunteers than in SMM patients suggesting a protection effect of this variant towards melanoma development. Accordingly, CARD8 expression was found to be reduced in nevus compared to melanoma biopsies. Upon stratification, NLRP1 rs11651270 and CARD8 rs2043211 were found associated with nodular melanoma; IL1B rs1143643 to a lower value of Breslow index; IL18 rs5744256 to melanoma development in sun sensitive individuals. As expected, IL1B expression was up-regulated in tumour biopsies especially in metastatic samples, whereas IL18 was down-regulated compared to nevus. Our results demonstrated for the first time the contribution of inflammasome genes CARD8, IL1B and IL18 in SMM. (AU)

Processo FAPESP: 13/06142-1 - Perfil genômico da resposta imune ao HIV-1 e implicações para a vacina terapêutica com células dendríticas contra o HIV-1
Beneficiário:Alessandra Pontillo
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/18448-5 - Análise do inflamassoma em melanoma: correlação entre células tumorais e resposta imune mediada por células dendríticas
Beneficiário:Wanessa Cardoso da Silva
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 12/09824-3 - Análise do inflamassoma em melanoma: correlação entre células tumorais e resposta imune mediada por células dendríticas
Beneficiário:Telma Miyuki Oshiro Sumida
Modalidade de apoio: Auxílio à Pesquisa - Regular