Anthropogenic impact on mangrove sediments triggers differential responses in the heavy metals and antibiotic resistomes of microbial communities

Autor(es)

Cabral, Lucelia ; Lacerda Junior, Gileno Vieira ; Pereira de Sousa, Sanderson Tarciso ; Franco Dias, Armando Cavalcante ; Cadete, Luana Lira ; Andreote, Fernando Dini ; Hess, Matthias ; de Oliveira, Valeria Maia

• Tipo de documento: Artigo Científico
• Fonte: Environmental Pollution; v. 216, p. 460-469, SEP 2016.
• Citações Web of Science: 17

Resumo

Mangroves are complex and dynamic ecosystems highly dependent on diverse microbial activities. In the last decades, these ecosystems have been exposed to and affected by diverse human activities, such as waste disposal and accidental oil spills. Complex microbial communities inhabiting the soil and sediment of mangroves comprise microorganisms that have developed mechanisms to adapt to organic and inorganic contaminants. The resistance of these microbes to contaminants is an attractive property and also the reason why soil and sediment living microorganisms and their enzymes have been considered promising for environmental detoxification. The aim of the present study was to identify active microbial genes in heavy metals, i.e., Cu, Zn, Cd, Pb and Hg, and antibiotic resistomes of polluted and pristine mangrove sediments through the comparative analysis of metatranscriptome data. The concentration of the heavy metals Zn, Cr, Pb, Cu, Ni, Cd, and Hg and abundance of genes and transcripts involved in resistance to toxic compounds (the cobalt-zinc-cadmium resistance protein complex; the cobalt -zinc cadmium resistance protein CzcA and the cation efflux system protein CusA) have been closely associated with sites impacted with petroleum, sludge and other urban waste. The taxonomic profiling of metatranscriptome sequences suggests that members of Gammaproteobacteria and Deltaproteobacteria classes contribute to the detoxification of the polluted soil. Desulfobacterium autotrophicum was the most abundant microorganism in the oil-impacted site and displayed specific functions related to heavy metal resistance, potentially playing a key role in the successful persistence of the microbial community of this site. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/16850-0 - Análise da diversidade funcional e bioprospecção de genes de degradação de hidrocarbonetos e de transformação de metais pesados em sedimentos de manguezal
• Beneficiário: Lucélia Cabral
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/50809-5 - Prospecção de metagenoma microbiano de sedimentos de manguezais na busca por novos compostos bioativos
• Beneficiário: Valeria Maia Merzel
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 13/20670-0 - Análise da diversidade funcional e bioprospecção de genes de degradação de hidrocarbonetos e de transformação de metais pesados em sedimentos de manguezal
• Beneficiário: Lucélia Cabral
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 12/06245-2 - Metatranscriptômica e contexto genômico de genes microbianos relacionados aos ciclos biogeoquímicos em manguezais
• Beneficiário: Fernando Dini Andreote
• Modalidade de apoio: Auxílio à Pesquisa - Regular