Anthropogenic impact on mangrove sediments triggers differential responses in the heavy metals and antibiotic resistomes of microbial communities

Author(s)

Cabral, Lucelia ; Lacerda Junior, Gileno Vieira ; Pereira de Sousa, Sanderson Tarciso ; Franco Dias, Armando Cavalcante ; Cadete, Luana Lira ; Andreote, Fernando Dini ; Hess, Matthias ; de Oliveira, Valeria Maia

• Document type: Journal article
• Source: Environmental Pollution; v. 216, p. 460-469, SEP 2016.
• Web of Science Citations: 17

Abstract

Mangroves are complex and dynamic ecosystems highly dependent on diverse microbial activities. In the last decades, these ecosystems have been exposed to and affected by diverse human activities, such as waste disposal and accidental oil spills. Complex microbial communities inhabiting the soil and sediment of mangroves comprise microorganisms that have developed mechanisms to adapt to organic and inorganic contaminants. The resistance of these microbes to contaminants is an attractive property and also the reason why soil and sediment living microorganisms and their enzymes have been considered promising for environmental detoxification. The aim of the present study was to identify active microbial genes in heavy metals, i.e., Cu, Zn, Cd, Pb and Hg, and antibiotic resistomes of polluted and pristine mangrove sediments through the comparative analysis of metatranscriptome data. The concentration of the heavy metals Zn, Cr, Pb, Cu, Ni, Cd, and Hg and abundance of genes and transcripts involved in resistance to toxic compounds (the cobalt-zinc-cadmium resistance protein complex; the cobalt -zinc cadmium resistance protein CzcA and the cation efflux system protein CusA) have been closely associated with sites impacted with petroleum, sludge and other urban waste. The taxonomic profiling of metatranscriptome sequences suggests that members of Gammaproteobacteria and Deltaproteobacteria classes contribute to the detoxification of the polluted soil. Desulfobacterium autotrophicum was the most abundant microorganism in the oil-impacted site and displayed specific functions related to heavy metal resistance, potentially playing a key role in the successful persistence of the microbial community of this site. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 12/06245-2 - Metatranscriptomics and genomic context of microbial genes related to biogeochemical cycles in mangroves
• Grantee: Fernando Dini Andreote
• Support type: Regular Research Grants
• FAPESP's process: 12/16850-0 - Assessment of the functional diversity and bioprospection of genes involved in degradation of hydrocarbons and transformation of heavy metals in mangrove sediments
• Grantee: Lucélia Cabral
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 13/20670-0 - Assessment of the functional diversity and bioprospection of genes involved in degradation of hydrocarbons and transformation of heavy metals in mangrove sediments
• Grantee: Lucélia Cabral
• Support type: Scholarships abroad - Research Internship - Post-doctor