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Metatranscriptomics and genomic context of microbial genes related to biogeochemical cycles in mangroves

Grant number: 12/06245-2
Support type:Regular Research Grants
Duration: August 01, 2012 - July 31, 2014
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Fernando Dini Andreote
Grantee:Fernando Dini Andreote
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Among the terrestrial ecosystems, some call our attention due to the particular combination of environmental parameter, resulting in the evolution of specie capable to colonize restrictively such environments. mangroves constitute a biome composed by species of plants, animals and microorganisms which interact in an environment characterized by occurrence in the interface between land and sea in inter-tropical regions. Such location doe confer unique conditions to this niche, mainly related with salinity, frequent anaerobiosis and seasonal variations in the availability of nutrients. In mangroves, bacteria, archaea and fungi constitute 91% of the total biomass; however, little is known about the functioning of the microbial groups in mangroves. In this way, this proposal aim to study by innovative technologies (metatranscriptomics and genomic context analysis) the functional microbial communities in two mangroves located in the State of Sao Paulo (already targeted by our group in previous studies). The gene expression will be determined by the massive sequencing of RNA (Illumina technology), aiming to identify genes expressed by the resident microbial community under distinct stages of mangroves preservation. Concerning the genomic context analysis, it will be performed the detection of genes related with biogeochemical cycling of carbon (mcrA), nitrogen (hzo, nifD, nifH, nirK, nirS, nosZ) and sulfur (aprA, dsrB), in metagenomic libraries already obtained with DNA extracted from the targeted mangroves (with 20Kb average for inserts sizes). It will support on inferences linking the phylogeny to function in the studied area. In sum, information will be collected about these functional communities in an innovative way, not only describing a single gene, but its expression in mangroves, and the genomic context in which does occur in the microbial community residing in this environment. (AU)

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CABRAL, LUCELIA; NORONHA, MELLINE FONTES; PEREIRA DE SOUSA, SANDERSON TARCISO; LACERDA-JUNIOR, GILENO VIEIRA; RICHTER, LARISSA; FOSTIER, ANNE HELENE; ANDREOTE, FERNANDO DINI; HESS, MATTHIAS; DE OLIVEIRA, VALERIA MAIA. The metagenomic landscape of xenobiotics biodegradation in mangrove sediments. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 179, p. 232-240, SEP 15 2019. Web of Science Citations: 0.
COTTA, SIMONE RAPOSO; CADETE, LUANA LIRA; VAN ELSAS, JAN DIRK; ANDREOTE, FERNANDO DINI; FRANCO DIAS, ARMANDO CAVALCANTE. Exploring bacterial functionality in mangrove sediments and its capability to overcome anthropogenic activity. Marine Pollution Bulletin, v. 141, p. 586-594, APR 2019. Web of Science Citations: 0.
DANICE M. LUVIZOTTO; JULIANA E. ARAUJO; MICHELE DE CÁSSIA P. SILVA; ARMANDO C. F. DIAS; BEATE KRAFT; HALINA TEGETMEYE; MARC STROUS; FERNANDO D. ANDREOTE. The rates and players of denitrification, dissimilatory nitrate reduction to ammonia (DNRA) and anaerobic ammonia oxidation (anammox) in mangrove soils. Anais da Academia Brasileira de Ciências, v. 91, p. -, 2019.
LUVIZOTT, DANICE M.; ARAUJO, JULIANA E.; SILVA, MICHELE DE CASSIA P.; DIAS, ARMANDO C. F.; KRAFT, BEATE; TEGETMEYE, HALINA; STROUS, MARC; ANDREOTE, FERNANDO D. The rates and players of denitrification, dissimilatory nitrate reduction to ammonia (DNRA) and anaerobic ammonia oxidation (anammox) in mangrove soils. Anais da Academia Brasileira de Ciências, v. 91, n. 1 2019. Web of Science Citations: 0.
CABRAL, LUCELIA; PEREIRA DE SOUSA, SANDERSON TARCISO; LACERDA JUNIOR, GILENO VIEIRA; HAWLEY, ERIK; ANDREOTE, FERNANDO DINI; HESS, MATTHIAS; DE OLIVEIRA, VALERIA MAIA. Microbial functional responses to long-term anthropogenic impact in mangrove soils. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 160, p. 231-239, SEP 30 2018. Web of Science Citations: 6.
CABRAL, LUCELIA; LACERDA JUNIOR, GILENO VIEIRA; PEREIRA DE SOUSA, SANDERSON TARCISO; FRANCO DIAS, ARMANDO CAVALCANTE; CADETE, LUANA LIRA; ANDREOTE, FERNANDO DINI; HESS, MATTHIAS; DE OLIVEIRA, VALERIA MAIA. Anthropogenic impact on mangrove sediments triggers differential responses in the heavy metals and antibiotic resistomes of microbial communities. Environmental Pollution, v. 216, p. 460-469, SEP 2016. Web of Science Citations: 20.
JIMENEZ, DIEGO JAVIER; DINI-ANDREOTE, FRANCISCO; OTTONI, JULIA RONZELLA; DE OLIVEIRA, VALERIA MAIA; VAN ELSAS, JAN DIRK; ANDREOTE, FERNANDO DINI. Compositional profile of alpha/beta-hydrolase fold proteins in mangrove soil metagenomes: prevalence of epoxide hydrolases and haloalkane dehalogenases in oil-contaminated sites. MICROBIAL BIOTECHNOLOGY, v. 8, n. 3, p. 604-613, MAY 2015. Web of Science Citations: 7.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.