Dynamics of production and consumption of methane by active microbiota in Amazonian wetlands

• Grant number: 16/16687-3
• Support Opportunities: Research Program on Global Climate Change - Young Investigators
• Start date: March 01, 2017
• End date: December 31, 2019
• Field of knowledge: Biological Sciences - Ecology - Ecosystems Ecology
• Principal Investigator: Acacio Aparecido Navarrete
• Grantee: Acacio Aparecido Navarrete
• Host Institution: Centro de Ciências e Tecnologias para a Sustentabilidade (CCTS). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil
• Associated researchers: Janaina Braga do Carmo ; Rogério Hartung Toppa ; Vania Neu
• Associated scholarship(s): 18/15847-2 - Training in physicochemical analysis and determination of methane by gas chromatography from environmental samples from Amazonian wetlands, BP.TT; 17/06415-9 - Dry and flood dynamics of methanothophs and methanogens on floodplains from the Negro and Solimões rivers in the Brazilian Amazon, BP.MS; 17/03575-5 - Dynamics of production and consumption of methane by active microbiota in Amazonian wetlands, BP.JP.PFPMCG

Abstract

Amazonian wetlands alone appear to contribute about 4% of the global annual emissions of CH4 from all natural and anthropogenic source according to published studies for over 10 years by the research team conducting the Large-scale Biosphere-Atmosphere Experiment in Amazônia (LBA). Given that microorganisms mediate the CH4 cycling, it is surprising that the role of microbial community composition in regulating the metabolic processes involved in CH4 flow in ecosystem scale is unknown. In this sense, this proposal aims to understand how the composition of methanogenic and methanotrophic microbial communities and their metabolic activities are associated with the production and consumption of CH4, and to investigate how the temperature and biogeochemistry, critical components to model the 'microbiota' component, affect the CH4 flow in the Amazon region. Latest generation technologies in metatranscriptomic and biogeochemical analyses will be used to investigate the relationship between methanogenic and methanotrophic microbial communities and production and consumption of CH4 in both natural and agricultural wetlands in the Brazilian Amazon (in situ studies), and under controlled conditions (biological reactors), that under temperature rise will allow identify the key parameters to delineate mathematical equations to be used for modeling the 'microbiota' component in CH4 flux in the Amazonia. Two different approaches will be used: 1) distinct isotopic signatures of production and consumption of CH4 will be used to relate changes in the spatial and temporal dynamics of CH4 in both natural and agricultural wetlands in floodplains of Amazonian rivers of black (Negro River), white (Solimões River) and clearwater (Tocantins River) in contrasting hydrograph (wet and dry seasons) with methanogenic and methanotrophic microbial communities and their metabolic activities revealed by metatranscriptomic approaches; 2) laboratory reactor test containing sediment from wetlands in floodplains of Amazon rivers of black, white and clearwater will be used to assessing the effect of temperature increase on the methanogenic and metanotrophic potential and metabolic activity of autochthonous microbial communities under controlled condition. The results will contribute significantly to the evaluation of hypotheses about the relationship between the microbiota and the production and consumption of CH4 in tropical wetlands, used here as an indicator of climate feedbacks related to emissions of gases causing the greenhouse effect. The research project presents an organized initiative in order to use the available infrastructure at CCTS-UFSCar and implement modern methods (analytical and computational/bioinformatics) and use new technologies for molecular and biogeochemical analysis in top-down and bottom-up perspectives of ecosystems supported by geoprocessing. The experience for use of the following methods: DNA/RNA high throughput sequencing, functional genes quantification using DNA microarrays (GeoChip), bioinformatics applied to 'omics' data analysis and metadata, and analysis of greenhouse gas emissions was acquired during the PhD and postdoctoral research developed by the principal investigator at University of São Paulo (USP), Netherlands Institute of Ecology (NIOO-Holanda), Institute for Environmental Genomics (IEG-EUA), Computational Science Research Center (SDSU-EUA) and University of Texas (UTA-EUA). Based on this proposal, it is expected ensure innovation in the field of research lines already implemented at CCTS-UFSCar and establish a new group/line of research in Environmental Genomics and Microbial Ecology, and place such groups of research from CCTS in the forefront of the studies on ecological implications of microorganisms in biogeochemical cycles... (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BENTO, MARILIA DE S.; BARROS, DAYANE J.; ARAUJO, MARIA GABRIELLA DA S.; DA ROZ, RAFAEL; CARVALHO, GLAUBER ALTRAO; DO CARMO, JANAINA B.; TOPPA, ROGERIO H.; NEU, VANIA; FORSBERG, BRUCE R.; BODELIER, PAUL L. E.; et al. Active methane processing microbes and the disproportionate role of NC10 phylum in methane mitigation in Amazonian floodplains. BIOGEOCHEMISTRY, v. 156, n. 2, SEP 2021. (17/03575-5, 17/06415-9, 18/02277-3, 16/16687-3, 18/15847-2)

GABRIEL, GABRIELE V. M.; OLIVEIRA, LUCIANA C.; BARROS, DAYANE J.; BENTO, MARILIA S.; NEU, VANIA; TOPPA, ROGERIO H.; CARMO, JANAINA B.; NAVARRETE, ACACIO A.. Methane emission suppression in flooded soil from Amazonia. Chemosphere, v. 250, JUL 2020. (17/03575-5, 12/50694-6, 16/16687-3, 13/50940-0, 16/10796-5)

NISHISAKA, CAROLINE SAYURI; YOUNGERMAN, CONNOR; MEREDITH, LAURA K.; DO CARMO, JANAINA BRAGA; NAVARRETE, ACACIO APARECIDO. Differences in N2O Fluxes and Denitrification Gene Abundance in the Wet and Dry Seasons Through Soil and Plant Residue Characteristics of Tropical Tree Crops. FRONTIERS IN ENVIRONMENTAL SCIENCE, v. 7, FEB 5 2019. (16/15289-4, 16/16687-3, 12/50694-6, 17/17441-0)

DE MELLO GABRIEL, GABRIELE VERONICA; PITOMBO, LEONARDO MACHADO; TAVARES ROSA, LUANA MARIA; NAVARRETE, ACACIO APARECIDO; BOTERO, WANDER GUSTAVO; DO CARMO, JANAINA BRAGA; DE OLIVEIRA, LUCIANA CAMARGO. The environmental importance of iron speciation in soils: evaluation of classic methodologies. ENVIRONMENTAL MONITORING AND ASSESSMENT, v. 193, n. 2, JAN 15 2021. (13/50940-0, 17/03575-5, 16/16687-3, 16/10796-5)

BARROS, DAYANE J.; CARVALHO, GLAUBER A.; DE CHAVES, MIRIAM G.; VANZELA, LUIZ S.; KOZUSNY-ANDREANI, DORA INES; GUARDA, EMERSON A.; NEU, VANIA; DE MORAIS, PAULA B.; TSAI, SIU M.; NAVARRETE, ACACIO A.. Microbial metabolic activity in Amazon floodplain forest and agricultural soils. FRONTIERS IN MICROBIOLOGY, v. 14, p. 12-pg., 2023-05-24. (17/03575-5, 16/16687-3)