Spatio-temporal dynamics of the active microbiota on the methane cycling in an Amazonian soil after forest-to-pasture conversion

• Grant number: 16/24695-6
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): March 01, 2017
• Status: Discontinued
• Field of knowledge: Biological Sciences - Ecology - Ecosystems Ecology
• Acordo de Cooperação: NSF - Dimensions of Biodiversity and BIOTA
• Principal Investigator: Tsai Siu Mui
• Grantee: Dasiel Obregón Alvarez
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 14/50320-4 - Dimensions US-BIOTA - São Paulo: collaborative research: integrating dimensions of microbial biodiversity across land use change in tropical forests, AP.BTA.TEM
• Associated scholarship(s): 18/05223-1 - Linking methane flux and microbial diversity in Amazon soils across forests-to-pasture conversion: a multidisciplinary approach, BE.EP.DD

Abstract

The Amazon watershed constitutes 40% of the world's rainforest, participating in global climate regulation and atmospheric gas-flow, including greenhouse gases (CO2, CH4, N2O). More than 15% of the Amazonian forest have been converted into agricultural and livestock areas, especially about 80% are occupied with pasture. The forest-to-pasture conversion have impacted on the soil microbiota and its relationships with the climate. This work aims to evaluate the impact of forest-to-pasture conversion in the dynamics of consumption and production of CH4 by the active microbiota of an Amazonian soil. For this purpose, biogeochemical, molecular and isotopic methods will be combined for the characterization of methanogenic (acetoclastic and hydrogenotrophic) and methanotrophic microbial communities, and their relationships with CH4 flow. It is proposed a spatial (biogeographic) and temporal (seasonal rainfall) approach in soils under primary forest, and converted to pastures of different ages in the Brazilian Western Amazon. Auto chamber for CH4 flux measurement, connected to a quantum cascade laser spectrometer (real-time isotopic analysis), along with massive DNA sequencing will provide unprecedent data for Amazonian soils, which will be combined using bioinformatics and statistical methods. It is expected that the results of this study will elucidate in more detail the role of microbial communities involved in the production and consumption of CH4 in soils under different uses in the Amazon region, integrating molecular and isotopic analyzes. These results may support new management strategies in tropical soils such as those from Amazonia, seeking for mitigation of CH4 emissions. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOLTANGHEISI, AMIN; DE MORAES, MOACIR TUZZIN; CHERUBIN, MAURICIO ROBERTO; ALVAREZ, DASIEL OBREGON; DE SOUZA, LEANDRO FONSECA; BIELUCZYK, WANDERLEI; NAVROSKI, DEISI; BETTONI TELES, ANA PAULA; PAVINATO, PAULO SERGIO; MARTINELLI, LUIZ ANTONIO; et al. Forest conversion to pasture affects soil phosphorus dynamics and nutritional status in Brazilian Amazon. SOIL & TILLAGE RESEARCH, v. 194, NOV 2019. (17/18327-7, 16/24695-6, 17/11332-5, 14/50320-4)

ALVAREZ, DASIEL OBREGON; DE SOUZA, LEANDRO FONSECA; MENDES, LUCAS WILLIAM; DE MORAES, MOACIR TUZZIN; TOSI, MICAELA; VENTURINI, ANDRESSA MONTEIRO; MEYER, KYLE M.; DE CAMARGO, PLINIO BARBOSA; BOHANNAN, BRENDAN J. M.; MAZZA RODRIGUES, JORGE L.; et al. Shifts in functional traits and interactions patterns of soil methane-cycling communities following forest-to-pasture conversion in the Amazon Basin. Molecular Ecology, v. 32, n. 12, p. 19-pg., 2023-03-21. (15/13546-7, 14/50320-4, 16/24695-6)

OBREGON, DASIEL; MAFA-ATTOYE, TOLULOPE G.; BASKERVILLE, MEGAN; MITTER, EDUARDO K.; DE SOUZA, LEANDRO FONSECA; OELBERMANN, MAREN; THEVATHASAN, NARESH V.; TSAI, SIU MUI; DUNFIELD, KARI E.. Functionality of methane cycling microbiome during methane flux hot moments from riparian buffer systems. Science of The Total Environment, v. 870, p. 12-pg., 2023-02-09. (18/09117-1, 14/50320-4, 18/05223-1, 16/24695-6)

ALVAREZ, DASIEL OBREGON; MENDES, KASSIO FERREIRA; TOSI, MICAELA; DE SOUZA, LEANDRO FONSECA; CAMPOS CEDANO, JOHNNY CARLOS; DE SOUZA FALCAO, NEWTON PAULO; DUNFIELD, KARI; TSAI, SIU MUI; TORNISIELO, VALDEMAR LUIZ. Sorption-desorption and biodegradation of sulfometuron-methyl and its effects on the bacterial communities in Amazonian soils amended with aged biochar. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 207, JAN 1 2021. (16/17683-1, 16/24695-6)