Microbial community resilience capacity assessed by metagenomics and metatranscriptomics in Amazon forest and pasture soils

• Grant number: 15/13546-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2015
• End date: June 30, 2019
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Tsai Siu Mui
• Grantee: Andressa Monteiro Venturini
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 14/50320-4 - Dimensions US-BIOTA - São Paulo: collaborative research: integrating dimensions of microbial biodiversity across land use change in tropical forests, AP.BTA.TEM
• Associated scholarship(s): 17/09643-2 - Soil microbial communities in forest-to-pasture conversion: a multi-omics approach, BE.EP.DR

Abstract

The Amazon Basin is covered by the largest rainforest on the planet, which has been converted into areas designated for agriculture due to the growing world demand for food. Land use change alters its original physical, chemical and biological characteristics. Consequently, the soil microorganisms, which participate in essential processes for all terrestrial ecosystems, respond differently to these changes, in a way to alter its taxonomic and functional diversity. Anthropic land use is considered as one of the main sources of greenhouse gases emissions to the atmosphere and thus the study of the effects of these changes in the microbial groups that act within the cycles of these gases is very important. Among these, the biogeochemical cycle of methane stands out, considered directly related to the soil microbial community attributes. Different environmental factors affect the groups of microbial methane producers and consumers, with studies that indicate strong moisture influence on them. However, the effects of land use change and moisture on soils under different uses on the taxonomic and functional microbial diversity have been little explored in the literature, especially in tropical soils. In this context, the combined use of molecular techniques from the total soil DNA and RNA can bring new data about these impacts on it. Therefore, the aim of the research project is to determine the effect of land use change and moisture in soils under different uses on its microbiota, emphasizing the methane cycle, through large-scale sequencing and quantitative real-time PCR of the total soil DNA and RNA from areas of primary forest, secondary forest and pasture in the State of Pará, in the eastern Amazon.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MANDRO, JESSICA A.; NAKAMURA, FERNANDA M.; GONTIJO, JULIA B.; TSAI, SIU M.; VENTURINI, ANDRESSA M.. Metagenome-Assembled Genomes from Amazonian Soil Microbial Consortia. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 11, n. 11, p. 5-pg., 2022-10-27. (14/50320-4, 15/12282-6, 15/23758-1, 15/13546-7, 19/26029-1, 18/14974-0, 19/25931-3)

GONTIJO, JULIA B.; PAULA, FABIANA S.; VENTURINI, ANDRESSA M.; YOSHIURA, CAIO A.; BORGES, CLOVIS D.; MOURA, JOSE MAURO S.; BOHANNAN, BRENDAN J. M.; NUSSLEIN, KLAUS; MAZZA RODRIGUES, JORGE L.; TSAI, SIU M.. Not just a methane source: Amazonian floodplain sediments harbour a high diversity of methanotrophs with different metabolic capabilities. Molecular Ecology, v. 30, n. 11, p. 2560-2572, JUN 2021. (14/50320-4, 17/26138-0, 18/14974-0, 17/09643-2, 15/13546-7, 19/25931-3)

ALVAREZ, DASIEL OBREGON; DE SOUZA, LEANDRO FONSECA; MENDES, LUCAS WILLIAM; DE MORAES, MOACIR TUZZIN; TOSI, MICAELA; VENTURINI, ANDRESSA MONTEIRO; MEYER, KYLE M.; DE CAMARGO, PLINIO BARBOSA; BOHANNAN, BRENDAN J. M.; MAZZA RODRIGUES, JORGE L.; et al. Shifts in functional traits and interactions patterns of soil methane-cycling communities following forest-to-pasture conversion in the Amazon Basin. Molecular Ecology, v. 32, n. 12, p. 19-pg., 2023-03-21. (15/13546-7, 14/50320-4, 16/24695-6)

LEMOS, LEANDRO NASCIMENTO; MANOHARAN, LOKESHWARAN; MENDES, LUCAS WILLIAM; VENTURINI, ANDRESSA MONTEIRO; PYLRO, VICTOR SATLER; TSAI, SIU MUI. Metagenome assembled-genomes reveal similar functional profiles ofCPR/Patescibacteria phyla in soils. ENVIRONMENTAL MICROBIOLOGY REPORTS, v. 12, n. 6, p. 651-655, DEC 2020. (17/24037-1, 17/09643-2, 14/50320-4, 16/18215-1, 15/13546-7)

YOSHIURA, CAIO AUGUSTO; VENTURINI, ANDRESSA MONTEIRO; PEREZ BRAGA, LUCAS PALMA; DA FRANCA, ALINE GIOVANA; CATANHO PEREIRA DE LYRA, MARIA DO CARMO; TSAI, SIU MUI; RODRIGUES, JORGE LUIZ MAZZA. Responses of Low-Cost Input Combinations on the Microbial Structure of the Maize Rhizosphere for Greenhouse Gas Mitigation and Plant Biomass Production. FRONTIERS IN PLANT SCIENCE, v. 12, JUN 30 2021. (13/22845-2, 15/13546-7, 15/08564-6, 16/11268-2, 14/50320-4, 19/25931-3)

VENTURINI, ANDRESSA MONTEIRO; NAKAMURA, FERNANDA MANCINI; GONTIJO, JULIA BRANDAO; DA FRANCA, ALINE GIOVANA; YOSHIURA, CAIO AUGUSTO; MANDRO, JESSICA ADRIELE; TSAI, SIU MUI. Robust DNA protocols for tropical soils. HELIYON, v. 6, n. 5, MAY 2020. (15/12282-6, 15/08564-6, 15/13546-7, 18/14974-0, 14/50320-4, 15/23758-1)

VENTURINI, ANDRESSA M.; DIAS, NAISSA M. S.; GONTIJO, JULIA B.; YOSHIURA, CAIO A.; PAULA, FABIANA S.; MEYER, KYLE M.; NAKAMURA, FERNANDA M.; DA FRANCA, ALINE G.; BORGES, CLOVIS D.; BARLOW, JOS; et al. Increased soil moisture intensifies the impacts of forest-to-pasture conversion on methane emissions and methane-cycling communities in the Eastern Amazon. Environmental Research, v. 212, p. 13-pg., 2022-04-06. (14/50320-4, 15/13546-7, 15/08564-6, 19/25931-3, 17/09616-5, 17/26138-0, 16/11268-2, 17/09643-2, 17/09952-5, 15/12282-6, 18/14974-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VENTURINI, Andressa Monteiro. Forest-to-pasture conversion in the Eastern Amazon: impacts on the soil methane microbial communities. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB) Piracicaba.