Microbial networks in control of greenhouse gases emissions from soil with sugarcane as affected by regular or concentrate vinasse

• Grant number: 14/24141-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 01, 2015
• End date: April 30, 2016
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Heitor Cantarella
• Grantee: Késia Silva Lourenço
• Supervisor: Eiko Eurya Kuramae
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Institution abroad: Royal Netherlands Academy of Arts and Sciences, Netherlands
• Associated to the scholarship: 13/12716-0 - GREENHOUSE GAS FLOWS (CO2, N2O AND CH4) FROM SOIL WITH SUGARCANE AS AFFECTED BY REGULAR OR CONCENTRATE VINASSE AT DIFFERENT APPLICATION TIMES., BP.DR

Abstract

Nitrogen (N) fertilization has been the main source of greenhouse gases (GHG) emissions to the atmosphere in sugarcane cultivation, mainly due to nitrous oxide (N2O) emission. In addition, some management practices of sugarcane production in Brazil may affect GHG emissions, such as the use of vinasse, a by-product of ethanol production. Recent studies indicate that the application of vinasse - especially with N fertilizers addition - results in significant increases of N2O emissions. Alternatives to reduce these emissions are necessary because the main destination of vinasse is the soil, where it recycles nutrients and improve soil fertility. Understanding the in-field GHG emissions in sugarcane under different vinasse management is important to ensure sugarcane ethanol sustainability. Our hypothesis is that in-depth knowledge the composition and functioning of microbial networks is important for the effects of vinasse on greenhouse gas production. Understanding such complex biotic and abiotic interactions is essential to develop sustainable sugarcane production including waste recycling as a component of the management system. The objective of this study is to determine the microbial functional groups, microbial functional N and C cycle genes and correlate with greenhouse gases (N2O, CO2 and CH4) emissions in soils with different sources and types of sugarcane residues. The experiment consisted (PhD Project - FAPESP 2013/12716-0) of two application times of vinasse, T1: 30 days before N fertilization and T2: together with N fertilization, and two types of vinasse (regular vinasse -RV or concentrated vinasse- CV), amounting to 10 treatments and four replications. Nitrogen was added at a rate of 100 kg ha-1 (ammonium nitrate) and vinasse at 100 m3 ha-1 for RV and 17 m3 ha-1 for CV, respectively. In parallel, for each sampling for gases measurements, the air and soil temperatures were evaluated and soil samples were taken for soil moisture, concentrations of N-NO3- and N-NH4+ (field studies in Brazil) and for nucleic acids extractions (to be done at NIOO). With this proposal we will better understand the interactive role of the microbial networks operating in soil and the consequences of bio-based agricultural management practices for the functioning of the soil ecosystem. IAC group members have strong expertise in soil sciences, sugarcane nitrogen nutrition (N-fixing bacteria and fertilizer), bioenergy wastes recycling, soil fertility, bioenergy crop management, plant nutrition and greenhouse gases emissions, whereas the NIOO group members have strong expertise in soil ecology, microbial ecology, ecological genomics, molecular biology, metagenomics, metatranscriptomics and bioinformatics. Therefore, the exchange of PhD students is fundamental not only for the success of the project but also give them a thorough education opportunity, given the complementarity of the both country's research teams. This PhD project will be linked to the project entitled "Microbial Networks in control of greenhouse gases emissions in Bio-based agriculture - MiniBag" (FAPESP-NWO 2013/50365-5) and "Flux of greenhouse gases (CO2, N2O E CH4) from soils with sugarcane as affected by fertilization and application of vinasse in different times" (FAPESP 2013/12716-0). (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SULEIMAN, AFNAN KHALIL AHMAD; LOURENCO, KESIA SILVA; CLARK, CALLUM; LUZ, RONILDSON LIMA; RIBEIRO DA SILVA, GUSTAVO HENRIQUE; VET, LOUISE E. M.; CANTARELLA, HEITOR; FERNANDES, V, TANIA; KURAMAE, EIKO EURYA. From toilet to agriculture: Fertilization with microalgal biomass from wastewater impacts the soil and rhizosphere active microbiomes, greenhouse gas emissions and plant growth. RESOURCES CONSERVATION AND RECYCLING, v. 161, OCT 2020. (13/50351-4, 18/20698-6, 14/24141-5)

LOURENCO, KESIA SILVA; DE ASSIS COSTA, OHANA YONARA; CANTARELLA, HEITOR; KURAMAE, EIKO EURYA. Ammonia-oxidizing bacteria and fungal denitrifier diversity are associated with N2O production in tropical soils. SOIL BIOLOGY & BIOCHEMISTRY, v. 166, p. 13-pg., 2022-03-01. (18/20698-6, 13/50365-5, 14/24141-5)

LOURENCO, KESIA S.; DIMITROV, MAURICIO R.; PIJL, AGATA; SOARES, JOHNNY R.; DO CARMO, JANAINA B.; VAN VEEN, JOHANNES A.; CANTARELLA, HEITOR; KURAMAE, EIKO E.. Dominance of bacterial ammonium oxidizers and fungal denitrifiers in the complex nitrogen cycle pathways related to nitrous oxide emission. Global Change Biology Bioenergy, v. 10, n. 9, p. 645-660, SEP 2018. (13/50365-5, 13/12716-0, 14/24141-5)

LOURENCO, KESIA S.; CASSMAN, NORIKO A.; PIJL, AGATA S.; VAN VEEN, JOHANNES A.; CANTARELLA, HEITOR; KURAMAE, EIKO E.. Nitrosospira sp Govern Nitrous Oxide Emissions in a Tropical Soil Amended With Residues of Bioenergy Crop. FRONTIERS IN MICROBIOLOGY, v. 9, APR 10 2018. (13/50365-5, 13/12716-0, 14/24141-5)

CASSMAN, NORIKO A.; LOURENCO, KESIA S.; DO CARMO, JANAINA B.; CANTARELLA, HEITOR; KURAMAE, EIKO E.. Genome-resolved metagenomics of sugarcane vinasse bacteria. BIOTECHNOLOGY FOR BIOFUELS, v. 11, FEB 22 2018. (13/50365-5, 13/12716-0, 14/24141-5)

LOURENCO, KESIA SILVA; ROSSETTO, RAFFAELLA; VITTI, ANDRE CESAR; MONTEZANO, ZAQUEU FERNANDO; SOARES, JOHNNY RODRIGUES; SOUSA, RAFAEL DE MELO; DO CARMO, JANAINA BRAGA; KURAMAE, EIKO EURYA; CANTARELLA, HEITOR. Strategies to mitigate the nitrous oxide emissions from nitrogen fertilizer applied with organic fertilizers in sugarcane. Science of The Total Environment, v. 650, p. 11-pg., 2019-02-10. (13/50365-5, 14/24141-5, 13/12716-0)

LOURENCO, KESIA SILVA; ROSSETTO, RAFFAELLA; VITTI, ANDRE CESAR; MONTEZANO, ZAQUEU FERNANDO; SOARES, JOHNNY RODRIGUES; SOUSA, RAFAEL DE MELO; DO CARMO, JANAINA BRAGA; KURAMAE, EIKO EURYA; CANTARELLA, HEITOR. Strategies to mitigate the nitrous oxide emissions from nitrogen fertilizer applied with organic fertilizers in sugarcane. Science of The Total Environment, v. 650, n. 1, p. 1476-1486, FEB 10 2019. (14/24141-5, 13/12716-0, 13/50365-5)

LOURENCO, KESIA SILVA; SULEIMAN, AFNAN K. A.; PIJL, A.; VAN VEEN, J. A.; CANTARELLA, H.; KURAMAE, E. E.. Resilience of the resident soil microbiome to organic and inorganic amendment disturbances and to temporary bacterial invasion. MICROBIOME, v. 6, AUG 13 2018. (13/50365-5, 13/12716-0, 14/24141-5)

SULEIMAN, AFNAN KHALIL AHMAD; LOURENCO, KESIA SILVA; PITOMBO, LEONARDO MACHADO; MENDES, LUCAS WILLIAM; WURDIG ROESCH, LUIZ FERNANDO; PIJL, AGATA; CARMO, JANAINA BRAGA; CANTARELLA, HEITOR; KURAMAE, EIKO EURYA. Recycling organic residues in agriculture impacts soil-borne microbial community structure, function and N2O emissions. Science of The Total Environment, v. 631-632, p. 1089-1099, AUG 1 2018. (12/50694-6, 13/12716-0, 14/24141-5)

LOURENCO, KESIA SILVA; SULEIMAN, AFNAN KHALIL AHMAD; PIJL, AGATA; CANTARELLA, HEITOR; KURAMAE, EIKO EURYA. Dynamics and resilience of soil mycobiome under multiple organic and inorganic pulse disturbances. Science of The Total Environment, v. 733, SEP 1 2020. (13/50365-5, 14/24141-5, 18/20698-6)