Propolis modulates miRNAs involved in TLR-4 pathway, NF-B activation, cytokine production and in the bactericidal activity of human dendritic cells

Autor(es)

Conti, Bruno J. ; Santiago, Karina B. ; Cardoso, Eliza O. ; Freire, Paula P. ; Carvalho, Robson F. ; Golim, Marjorie A. ; Sforcin, Jose M.

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmacy and Pharmacology; v. 68, n. 12, p. 1604-1612, DEC 2016.
• Citações Web of Science: 7

Resumo

ObjectivesDendritic cells (DCs) are antigen-presenting cells, essential for recognition and presentation of pathogens to T cells. Propolis, a resinous material produced by bees from various plants, exhibits numerous biological properties, highlighting its immunomodulatory action. Here, we assayed the effects of propolis on the maturation and function of human DCs. MethodsDCs were generated from human monocytes and incubated with propolis and LPS. NF-B and cytokines production were determined by ELISA. microRNA's expression was analysed by RT-qPCR and cell markers detection by flow cytometry. Colony-forming units were obtained to assess the bactericidal activity of propolis-treated DCs. Key findingsPropolis activated DCs in the presence of LPS, inducing NF-kB, TNF-, IL-6 and IL-10 production. The inhibition of hsa-miR-148a and hsa-miR-148b abolished the inhibitory effects on HLA-DR and pro-inflammatory cytokines. The increased expression of hsa-miR-155 may be correlated to the increase in TLR-4 and CD86 expression, maintaining LPS-induced expression of HLA-DR and CD40. Such parameters may be involved in the increased bactericidal activity of DCs against Streptococcus mutans. ConclusionPropolis modulated the maturation and function of DCs and may be useful in the initial steps of the immune response, providing a novel approach to the development of DC-based strategies and for the discovery of new immunomodulators. (AU)

• Processo FAPESP: 12/13996-4 - Ação da própolis sobre a maturação e função de células dendríticas humanas
• Beneficiário: Bruno Jose Conti
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/14176-0 - Ação da própolis sobre a maturação e função de células dendríticas humanas
• Beneficiário: José Maurício Sforcin
• Modalidade de apoio: Auxílio à Pesquisa - Regular