Chemokine, cytokine and type I interferon production induced by Toll-like receptor activation in common variable immune deficiency

Autor(es)

de Lollo, Camila ; Vasconcelos, Dewton de Moraes ; da Silva Oliveira, Luanda Mara ; Domingues, Rosana ; de Carvalho, Gabriel Costa ; da Silva Duarte, Alberto Jose ; Sato, Maria Notomi

• Tipo de documento: Artigo Científico
• Fonte: Clinical Immunology; v. 169, p. 121-127, AUG 2016.
• Citações Web of Science: 2

Resumo

Common variable immunodeficiency (CVID) is the most common symptomatic primary antibody deficiency and is associated with recurrent infections and chronic inflammatory diseases. We evaluated the ability of Toll-like receptor (TLR) ligands to induce secretion of chemokines, cytokines and type I interferons by peripheral blood mononuclear cells (PBMCs) from CVID patients. High levels of CXCL10, CCL2, CXCL9, CCL5, CXCL8, and IL-6 were detected in sera of CVID patients compared with healthy controls. Increased chemokine levels were observed in unstimulated PBMCs, but after stimulation with TLR2 and TLR4 agonists, equivalent chemokine and pro-inflammatory cytokine secretion, as in healthy controls, was observed, whereas TLR4 agonist induced a decreased secretion of CCL2 and CXCL8 and increased secretion of TNF. Decreased IFN-alpha secretion induced by TLR7/TLR8 activation was observed in CVID, which was recovered with TLR9 signaling. Our findings revealed that TLR9 activation has an adjuvant effect on the altered type I response in CVID. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/14110-0 - Avaliação das células T CD4+ polifuncionais e reguladoras na deficiência seletiva de IgA
• Beneficiário: Camila de Lollo
• Modalidade de apoio: Bolsas no Brasil - Mestrado