Chemokine, cytokine and type I interferon production induced by Toll-like receptor activation in common variable immune deficiency

Author(s)

de Lollo, Camila ; Vasconcelos, Dewton de Moraes ; da Silva Oliveira, Luanda Mara ; Domingues, Rosana ; de Carvalho, Gabriel Costa ; da Silva Duarte, Alberto Jose ; Sato, Maria Notomi

• Document type: Journal article
• Source: Clinical Immunology; v. 169, p. 121-127, AUG 2016.
• Web of Science Citations: 2

Abstract

Common variable immunodeficiency (CVID) is the most common symptomatic primary antibody deficiency and is associated with recurrent infections and chronic inflammatory diseases. We evaluated the ability of Toll-like receptor (TLR) ligands to induce secretion of chemokines, cytokines and type I interferons by peripheral blood mononuclear cells (PBMCs) from CVID patients. High levels of CXCL10, CCL2, CXCL9, CCL5, CXCL8, and IL-6 were detected in sera of CVID patients compared with healthy controls. Increased chemokine levels were observed in unstimulated PBMCs, but after stimulation with TLR2 and TLR4 agonists, equivalent chemokine and pro-inflammatory cytokine secretion, as in healthy controls, was observed, whereas TLR4 agonist induced a decreased secretion of CCL2 and CXCL8 and increased secretion of TNF. Decreased IFN-alpha secretion induced by TLR7/TLR8 activation was observed in CVID, which was recovered with TLR9 signaling. Our findings revealed that TLR9 activation has an adjuvant effect on the altered type I response in CVID. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 12/14110-0 - Evaluation of polyfunctional CD4+ T cells and regulatory in the selective IgA deficiency
• Grantee: Camila de Lollo
• Support Opportunities: Scholarships in Brazil - Master