Evaluation and characterization of plasmids carrying CTX-M genes in a non-clonal population of multidrug-resistant Enterobacteriaceae isolated from poultry in Brazil

Autor(es)

Ferreira, Joseane Cristina ; Casarin Penha Filho, Rafael Antonio ; Andrade, Leonardo Neves ; Berchieri Junior, Angelo ; Costa Darini, Ana Lucia

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 85, n. 4, p. 444-448, AUG 2016.
• Citações Web of Science: 11

Resumo

The increasing presence of ESBL-producing bacteria in food-producing animals might impact on public health. In this study, ESBL-producing enterobacteria were investigated in the microbiota of chickens produced in Brazil. We detected bla(CTX-M-2), bla(CTX-M-8) and bla(CTX-M-15) in 13 Escherichia coli isolates, within 9 different PFGE-types. Escherichia fergusonii and Klebsiella pneumoniae were found carrying bla(CTX-M-2). Plasmid Inc groups found included repF, FIB, FIC, I1, Y, B/O, A/C, K and HI1. F plasmids were present in 87.5% of the isolates, however, no resistance gene was harbored in this replicon. The pMLST for IncI1 showed ST113 and the novel ST130, ST131 and ST132 harboring bla(CTX-M-8). IncK plasmids carried bla(CTX-M-2) in one E. coli isolate. Non-typeable plasmids carrying bla(CTX-M-2) or bla(CTX-M-15) had up to 260 kb. bla(CTX-M-2) was also associated with class I integron and ISCR1 and bla(CTX-M-8) with IS10. Overall, similar resistance elements were disseminated among a diverse population of ESBL-producing enterobacteria. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/08892-2 - Investigação molecular de linhagens de Klebsiella pneumoniae e plasmídeos carreando genes codificadores de beta-lactamases
• Beneficiário: Leonardo Neves de Andrade
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/24017-7 - Avaliação da infecção e imunidade de linhagens de aves (Gallus Gallus) suscetíveis e resistentes ao desafio por Salmonella enterica subesp. enterica sorovar Gallinarum
• Beneficiário: Rafael Antonio Casarin Penha Filho
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/14494-8 - Epidemiologia molecular de bactérias gram-negativas e genética da resistência a antibióticos
• Beneficiário: Ana Lúcia da Costa Darini
• Linha de fomento: Auxílio à Pesquisa - Temático