Evaluation and characterization of plasmids carrying CTX-M genes in a non-clonal population of multidrug-resistant Enterobacteriaceae isolated from poultry in Brazil

Author(s)

Ferreira, Joseane Cristina ; Casarin Penha Filho, Rafael Antonio ; Andrade, Leonardo Neves ; Berchieri Junior, Angelo ; Costa Darini, Ana Lucia

• Document type: Journal article
• Source: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 85, n. 4, p. 444-448, AUG 2016.
• Web of Science Citations: 11

Abstract

The increasing presence of ESBL-producing bacteria in food-producing animals might impact on public health. In this study, ESBL-producing enterobacteria were investigated in the microbiota of chickens produced in Brazil. We detected bla(CTX-M-2), bla(CTX-M-8) and bla(CTX-M-15) in 13 Escherichia coli isolates, within 9 different PFGE-types. Escherichia fergusonii and Klebsiella pneumoniae were found carrying bla(CTX-M-2). Plasmid Inc groups found included repF, FIB, FIC, I1, Y, B/O, A/C, K and HI1. F plasmids were present in 87.5% of the isolates, however, no resistance gene was harbored in this replicon. The pMLST for IncI1 showed ST113 and the novel ST130, ST131 and ST132 harboring bla(CTX-M-8). IncK plasmids carried bla(CTX-M-2) in one E. coli isolate. Non-typeable plasmids carrying bla(CTX-M-2) or bla(CTX-M-15) had up to 260 kb. bla(CTX-M-2) was also associated with class I integron and ISCR1 and bla(CTX-M-8) with IS10. Overall, similar resistance elements were disseminated among a diverse population of ESBL-producing enterobacteria. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 14/14494-8 - Molecular epidemiology of gram-negative bacteria and genetic of antibiotic resistance
• Grantee: Ana Lúcia da Costa Darini
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/08892-2 - Molecular investigation of Klebsiella pneumoniae strains and plasmids carrying beta-lactamases encoding genes
• Grantee: Leonardo Neves de Andrade
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/24017-7 - Evaluation of the infection and immunity in susceptible and resistant lines of chicken (Gallus gallus) challenged with Salmonella enterica subsp. enterica sorovar Gallinarum
• Grantee: Rafael Antonio Casarin Penha Filho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral