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Molecular investigation of Klebsiella pneumoniae strains and plasmids carrying beta-lactamases encoding genes

Grant number: 11/08892-2
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): November 01, 2011
Effective date (End): May 31, 2015
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Ana Lúcia da Costa Darini
Grantee:Leonardo Neves de Andrade
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):13/26188-6 - Virulence factors and intraclonal diversity of ESBL and carbapenemase-producing Klebsiella pneumoniae and Escherichia coli clinical isolates, BE.EP.PD


Beta-lactamases-producing enterobacteria are a worldwide problem, observed mainly in the last two decades with the pandemic of beta-lactamases CTX-M and most recently the dissemination of KPC and emergence of NDM carbapenemases. In Brazil, few studies report with detail the molecular epidemiology of bacterial strains prevalent and/or epidemic plasmids that are involved in the spread of resistance. In this context, the aim of this project is to investigate K. pneumoniae isolates and plasmids carrying beta-lactamases encoding genes in the northeast region of São Paulo State - Brazil. Thus, isolates of broad-spectrum beta-lactam-resistant K. pneumoniae will be investigated. Beta-lactamases encoding genes will be investigated, especially those commonly carried by plasmids. The bacterial strains will be defined considering the macrorestriction profile by pulsed-field gel electrophoresis and, according to the sequence type, by Multilocus Sequence Typing. The characterization of plasmids will be accomplished by typing of incompatibility groups and hybridization. This knowledge will be fundamental in near future since new strategies can be formulated to control antibiotic resistance, more incisive and effective, directly in the chain of resistance genes spread.

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANDRADE, LEONARDO NEVES; NOVAIS, ANGELA; MARCATO STEGANI, LENITA MARIA; FERREIRA, JOSEANE CRISTINA; RODRIGUES, CARLA; COSTA DARINI, ANA LUCIA; PEIXE, LUISA. Virulence genes, capsular and plasmid types of multidrug-resistant CTXM(-2,-8,-15) and KPC-2-producing Klebsiella pneumoniae isolates from four major hospitals in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 91, n. 2, p. 164-168, . (12/14740-3, 11/08892-2, 13/26188-6)
GASPAR, GILBERTO GAMBERO; BELLISSIMO-RODRIGUES, FERNANDO; DE ANDRADE, LEONARDO NEVES; DARINI, ANA LUCIA; MARTINEZ, ROBERTO. Induction and nosocomial dissemination of carbapenem and polymyxin-resistant Klebsiella pneumoniae. Revista da Sociedade Brasileira de Medicina Tropical, v. 48, n. 4, p. 483-487, . (11/08892-2)
FERREIRA, JOSEANE CRISTINA; CASARIN PENHA FILHO, RAFAEL ANTONIO; ANDRADE, LEONARDO NEVES; BERCHIERI JUNIOR, ANGELO; COSTA DARINI, ANA LUCIA. Evaluation and characterization of plasmids carrying CTX-M genes in a non-clonal population of multidrug-resistant Enterobacteriaceae isolated from poultry in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 85, n. 4, p. 444-448, . (14/14494-8, 11/08892-2, 12/24017-7)
ANDRADE, LEONARDO NEVES; VITALI, LUCIA; GASPAR, GILBERTO GAMBERO; BELLISSIMO-RODRIGUES, FERNANDO; MARTINEZ, ROBERTO; COSTA DARINI, ANA LUCIA. Expansion and Evolution of a Virulent, Extensively Drug-Resistant (Polymyxin B-Resistant), QnrS1-, CTX-M-2-, and KPC-2-Producing Klebsiella pneumoniae ST11 International High-Risk Clone. Journal of Clinical Microbiology, v. 52, n. 7, p. 2530-2535, . (12/14740-3, 11/08892-2)

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