Differentiation of cocoa nibs from distinct origins using comprehensive two-dimensional gas chromatography and multivariate analysis

Autor(es)

Oliveira, Luciana F. ; Braga, Soraia C. G. N. ; Augusto, Fabio ; Hashimoto, Juliana C. ; Efraim, Priscilla ; Poppi, Ronei J.

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 90, p. 133-138, DEC 2016.
• Citações Web of Science: 13

Resumo

Headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography was used for identification of volatiles compounds responsible for differentiation between cocoa nibs from Brazil and Ivory Coast. The unfolded GC x GC with Flame Ionization Detector (FID) chromatograms were first aligned using the correlation optimization warping (COW) algorithm and normalized. After that, Fisher ratio was calculated for each variable, and a threshold value was chosen-to select the variables that best promote a separation of nibs samples from different sources in a principal component analysis (PCA) model. To identify the relevant compounds for the separation, representative samples of each source were analyzed in the same conditions by GC x GC with detection by quadrupole mass spectrometry. Finally, the average peak volumes for each key compound obtained for both classes were compared using a Student t-test and it was possible to identify 15 volatile compounds responsible for differentiation between cocoa nibs from Brazil and Ivory Coast. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/14695-8 - Emprego de métodos quimiométricos em análises por cromatografia gasosa bidimensional abrangente para avaliação de cacau, seus derivados e alfarroba
• Beneficiário: Luciana Fontes de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado