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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Role of NOD2 and RIP2 in host-microbe interactions with Gram-negative bacteria: insights from the periodontal disease model

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Autor(es):
Souza, Joao A. C. ; Medeiros, Marcell C. ; Rocha, Fernanda R. G. ; de Aquino, Sabrina G. ; Avila-Campos, Mario J. ; Spolidorio, Luis C. ; Zamboni, Dario S. ; Graves, Dana T. ; Rossa Junior, Carlos
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: Innate Immunity; v. 22, n. 8, p. 598-611, NOV 2016.
Citações Web of Science: 3
Resumo

NOD2 is a member of the NLR family of proteins that participate in the activation of the innate immune response. RIP2 is a downstream kinase activated by both NOD1 and NOD2. There is scarcity of information regarding the relevance of NOD2 in periodontitis, a chronic inflammatory condition characterized by inflammatory bone resorption. We used NOD2-KO and RIP2-KO mice in a model of microbial-induced periodontitis. Heat-killed Aggregatibacter actinomycetemcomitans was injected in the gingival tissues three times/wk for 4 wk. Bone resorption was assessed by CT analysis; osteoclasts were identified by immunohistochemical staining for TRAP and inflammation was assessed using a severity score system in H/E-stained sections. Invitro studies using primary macrophages assessed the response macrophages using qPCR-based array and multi-ligand ELISA. Bone resorption and osteoclastogenesis were significantly reduced in NOD2-KO mice. Severity of inflammation was not affected. qPCR-focused arrays and multi-ligand ELISA showed that expression of pro-inflammatory mediators was reduced in NOD2- and RIP2-deficient cells. RANKL-induced osteoclastogenesis was impaired in NOD2- and RIP2-deficient macrophages. We conclude that NOD2 is important for osteoclast differentiation and inflammatory bone resorption invivo and also for the macrophage response to Gram-negative bacteria. (AU)

Processo FAPESP: 10/05632-7 - Papel das proteínas Nod na modulação da resposta imune nas doenças periodontais
Beneficiário:João Antonio Chaves de Souza
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 10/05783-5 - Papel das proteínas Nod na modulação da resposta imune nas doenças periodontais
Beneficiário:Carlos Rossa Junior
Linha de fomento: Auxílio à Pesquisa - Regular