Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

Autor(es)

Marcelo Rodrigues da Cunha ; Fernanda Araujo Menezes ^[2] ; Geovane Ribeiro dos Santos ^[3] ; Clóvis Antônio Lopes Pinto ^[4] ; Benedito Barraviera ^[5] ; Virginia da Conceição Amaro Martins ^[6] ; Ana Maria de Guzzi Plepis ^[7] ; Rui Seabra Ferreira Junior ^[8]

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS; v. 18, n. 1, p. 196-203, 2015-02-00.

Resumo

Biomaterials are used as a promising alternative to bone grafts, including bioceramics whose composition resembles that of bone and fibrin sealants due to their hemostatic properties. The objective was to evaluate the repair of cranial defects in 40 rats, grafted with hydroxyapatite and a new fibrin sealant derived from snake venom. The animals were divided into four groups: C (control, no graft); Ha (hydroxyapatite); FS (fibrin sealant), and HaFS (hydroxyapatite and fibrin sealant). The animals were euthanized 2 and 6 weeks after surgery and wound area were submitted to analysis. After 2 weeks, immature bone was formed from the borders of the defect and in groups Ha and HaFS, few hydroxyapatite particles were surrounded by new bone. After 6 weeks, the new bone was mature and surrounded several hydroxyapatite particles, without connective tissue interposition and the volume of new bone was higher in HaFS group. The hydroxyapatite in combination with the new fibrin sealant accelerates bone repair. (AU)

• Processo FAPESP: 12/02689-3 - Utilização do selante de fibrina associado à hidroxiapatita no reparo de falhas ósseas provocadas na calvária de ratos
• Beneficiário: Fernanda de Araujo Menezes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/53846-9 - EMU: aquisição de cromatógrafo líquido de alta eficiência (CLAE) preparativo para purificação e isolamento de serinoproteinases para produção do selante de fibrina
• Beneficiário: Benedito Barraviera
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 09/06280-0 - Variação sexual, ontogenética e ambiental do veneno de Crotalus durissus terrificus e Bothrops jararaca da região de Botucatu - São Paulo: Caracterização enzimática, bioquímica e farmacológica
• Beneficiário: Rui Seabra Ferreira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/08101-8 - Imageamento in vivo da regeneração nervosa após reimplante de raízes motoras com selante de fibrina fluo-marcado associado com células tronco mesenquimais
• Beneficiário: Rui Seabra Ferreira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular