Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

Author(s)

Marcelo Rodrigues da Cunha ; Fernanda Araujo Menezes ^[2] ; Geovane Ribeiro dos Santos ^[3] ; Clóvis Antônio Lopes Pinto ^[4] ; Benedito Barraviera ^[5] ; Virginia da Conceição Amaro Martins ^[6] ; Ana Maria de Guzzi Plepis ^[7] ; Rui Seabra Ferreira Junior ^[8]

• Document type: Journal article
• Source: MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS; v. 18, n. 1, p. 196-203, 2015-02-00.

Abstract

Biomaterials are used as a promising alternative to bone grafts, including bioceramics whose composition resembles that of bone and fibrin sealants due to their hemostatic properties. The objective was to evaluate the repair of cranial defects in 40 rats, grafted with hydroxyapatite and a new fibrin sealant derived from snake venom. The animals were divided into four groups: C (control, no graft); Ha (hydroxyapatite); FS (fibrin sealant), and HaFS (hydroxyapatite and fibrin sealant). The animals were euthanized 2 and 6 weeks after surgery and wound area were submitted to analysis. After 2 weeks, immature bone was formed from the borders of the defect and in groups Ha and HaFS, few hydroxyapatite particles were surrounded by new bone. After 6 weeks, the new bone was mature and surrounded several hydroxyapatite particles, without connective tissue interposition and the volume of new bone was higher in HaFS group. The hydroxyapatite in combination with the new fibrin sealant accelerates bone repair. (AU)

• FAPESP's process: 12/08101-8 - In vivo imaging of nerve regeneration following ventral root replantation with fluo-stained fibrin sealant associated with mesenchymal stem cells
• Grantee: Rui Seabra Ferreira Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/02689-3 - Use of fibrin sealant associated with hydroxyapatite in repair of bone defects induced in rat calvaria
• Grantee: Fernanda de Araujo Menezes
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 09/06280-0 - Sexual, ontogenetic and enviromental variation in the venom of Crotalus durissus terrificus and Bothrops jararaca on Botucatu region - Sao Paulo State - Brazil: Pharmacological, Biochemical and Enzymatical Characterization.
• Grantee: Rui Seabra Ferreira Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/53846-9 - Recent acquisition of preparative high-performance liquid chromatograph for the purification and isolation of serine proteinases to produce fibrin sealant
• Grantee: Benedito Barraviera
• Support Opportunities: Multi-user Equipment Program