Identification of prognostic and diagnostics molecular biomarkers of cicatrization in the inflammatory exudate of chronic venous ulcers before and after treatment with fibrin sealant

• Grant number: 14/13299-7
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2014 - January 31, 2017
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Lucilene Delazari dos Santos
• Grantee: Lucilene Delazari dos Santos
• Host Institution: Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP). Centro Virtual de Toxinologia. Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Benedito Barraviera ; José-Luis Capelo-Martínez ; Luciana Patrícia Fernandes Abbade ; Rui Seabra Ferreira Junior

Abstract

Because of the identification of individual molecular signatures in biological systems by proteomic approach, there was an increase in the discovery of biomarkers that could be used in clinical therapy as well as in the development of prognostic/diagnostic tests. The Dermatology Ambulatory from Botucatu Medical School at UNESP treats patients with chronic venous ulcers weekly and, at the moment, it has no comprehension about ulcers healing and about determination of prognostic signatures. Currently, the recommended treatments for chronic venous ulcers are not differentiated, given that, the initial efforts to implement an effective therapy is compromised when there is no information about prognosis or stage of healing in these ulcers. Therefore, the patient reports and clinical aspects are the main tools capable of assessing the degree of difficulty and healing. Nowadays, a great cientific and tecnological challenge have been assumed by researchs from The Center for the Study of Venoms and Venomous Animals (CEVAP) at UNESP in partnership with the Postgraduate Program in Tropical Diseases of Botucatu Medical School (FMB/UNESP) through a clinical trial phase I/II to evaluate the safety and best dose of the new fibrin sealant derived from snake venom in the treatment of chronic venous ulcers, which is supported and funded by the Ministry of Science and Technology (Decit) and by CNPq proc. number. 563582/2010-3. Since, there is no biochemical diagnostic and prognostic methods to differentiate chronic venous ulcers of easy or difficult cicatrization, it was necessary to search for specific molecular markers through proteomic approach. The aim of this proposal is to identify the proteins present in inflammatory exudates of patients with chronic venous ulcers, in order to find specific molecular markers that can infer the stage of ulcer healing easy and difficult to heal, before and after the use of the new fibrin sealant CEVAP. This proposal has pretension to bridge the gap between discoveries at the bench and assistance patients at the bedside (from the beanch to the bedside). The future goals after the final analysis of the results will be: to suggest the insertion of proteomics analytical tools clinical routine in dermatology to have a precocious prognosis of ulcer healing; to transfer knowledge from basic research improving and creating new diagnostic methods to chronic venous ulcers; to establish therapeutic platforms capable of immediate clinical use, and, finally, to assist in the patenting of the new fibrin sealant, which is considered strategic and confidential at this moment. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LUCIANA PATRICIA FERNANDES ABBADE; RUI SEABRA FERREIRA JR; LUCILENE DELAZARI DOS SANTOS; BENEDITO BARRAVIERA. Chronic venous ulcers: a review on treatment with fibrin sealant and prognostic advances using proteomic strategies. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-06-22. (14/13299-7)

DE PONTES, LETICIA GOMES; VIEIRA CAVASSAN, NAYARA RODRIGUES; DE BARROS, LUCIANA CURTOLO; FERREIRA JUNIOR, RUI SEABRA; BARRAVIERA, BENEDITO; DOS SANTOS, LUCILENE DELAZARI. Plasma proteome of buffaloes. PROTEOMICS CLINICAL APPLICATIONS, v. 11, n. 9-10, SEP 2017. (14/13299-7, 12/08101-8)

LETICIA GOMES DE PONTES; WANESSA FERNANDA ALTEI; ASIER GALAN; PETRA BILI; NICOLAS GUILLEMIN; JOSIPA KULE; ANITA HORVATI; LÍGIA NUNES DE MORAIS RIBEIRO; ENEIDA DE PAULA; VIRGÍNIA BODELÃO RICHINI PEREIRA; et al. Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-05-29. (14/13299-7)

FERREIRA, JR., RUI SEABRA; DE BARROS, LUCIANA CURTOLO; FERNANDES ABBADE, LUCIANA PATIRCIA; CATHARINO SARTORI BARRAVIERA, SILVIA REGINA; CAVARIANI SILVARES, MARIA REGINA; DE PONTES, LETICIA GOMES; DOS SANTOS, LUCILENE DELAZARI; BARRAVIERA, BENEDITO. Heterologous fibrin sealant derived from snake venom: from bench to bedside - an overview. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 1, APR 4 2017. (09/53846-9, 12/08101-8, 14/13299-7)

ABBADE, LUCIANA P. F.; BARRAVIERA, SILVIA REGINA CATHARINO SARTORI; SILVARES, MARIA REGINA CAVARIANI; LIMA, ANA BEATRIZ B. DE C. O.; HADDAD, GABRIELA R.; GATTI, MARCIA A. N.; MEDOLAGO, NATALIA BRONZATTO; RIGOTTO CARNEIRO, MARCIA TONIN; DOS SANTOS, LUCILENE DELAZARI; FERREIRA, JR., RUI SEABRA; et al. Treatment of Chronic Venous Ulcers With Heterologous Fibrin Sealant: A Phase I/II Clinical Trial. FRONTIERS IN IMMUNOLOGY, v. 12, FEB 23 2021. (14/13299-7)

RUI SEABRA FERREIRA JR; LUCIANA CURTOLO DE BARROS; LUCIANA PATRÍCIA FERNANDES ABBADE; SILVIA REGINA CATHARINO SARTORI BARRAVIERA; MARIA REGINA CAVARIANI SILVARES; LETICIA GOMES DE PONTES; LUCILENE DELAZARI DOS SANTOS; BENEDITO BARRAVIERA. Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, 2017-07-06. (12/08101-8, 09/53846-9, 14/13299-7)

VIEIRA CAVASSAN, NAYARA RODRIGUES; CAMARGO, CAIO CAVASSAN; DE PONTES, LETICIA GOMES; BARRAVIERA, BENEDITO; FERREIRA, RUI SEABRA; MIOT, HELLO AMANTE; FERNANDES ABBADE, LUCIANA PATRICIA; DOS SANTOS, LUCILENE DELAZARI. Correlation between chronic venous ulcer exudate proteins and clinical profile: A cross-sectional study. JOURNAL OF PROTEOMICS, v. 192, p. 280-290, FEB 10 2019. (14/13299-7)