Evaluation of snake venom-derived fibrin sealant as a biological scaffold for mesenchymal stem cells in rat femur bone regeneration

• Grant number: 13/02004-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2013
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Rui Seabra Ferreira Junior
• Grantee: Camila Fernanda Zorzella Creste
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Despite the high regenerative capacity of bone tissue, the biologic process may fail or be limited by factors such as the size of the defect. Bone remodeling is a process that occurs in localized bone degradation, followed by deposition in equal quantity of bone material in the same location. Currently, stem cell therapy has shown promise in the treatment of experimental bone repair. In this sense, tissue engineering has emerged as a multidisciplinary field that aims to regenerate tissues and restore organ function by combining living cells with a synthetic or natural sacaffold to produce a three-dimensional living tissue that is functionally structurally and mechanically the same as the original tissue. The possibility of using a Fibrin Sealant, whose polymerizing factor is derived from snake venom, opens an important medical-surgical option. The advantages to conventional sealants available , not using human blood in their composition, the possibility of formulating customized to the need of surgery, taking into account the clotting time, as well as controlling the degradation time of Sealant . The possibility of using cells suspended in fibrin sealants, instead of commonly used solid scaffolds, allows rapid stabilization of these cells in suspension. This project aims to evaluate the influence of Fibrin Sealant derived from snake venom on mesenchymal stem cells (MSCs) non-differentiated and pre-differentiated in the repair of bone defects in rat femurs. Considering that the use of fibrin sealant can be applied as sacaffold for biological MSCs, so we can create alternative therapies in bone repair.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CRESTE, CAMILA FERNANDA ZORZELLA; ORSI, PATRICIA RODRIGUES; LANDIM-ALVARENGA, FERNANDA CRUZ; JUSTULIN, LUIS ANTONIO; GOLIM, MARJORIE DE ASSIS; BARRAVIERA, BENEDITO; FERREIRA, RUI SEABRA. Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration. MATERIALS, v. 13, n. 12, JUN 2020. (13/02004-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CRESTE, Camila Fernanda Zorzella. Avaliação do uso de Selante de Fibrina derivado do veneno de serpente como arcabouço biológico de células tronco mesenquimais na regeneração óssea do fêmur de ratos. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu Botucatu.