Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration

Author(s)

Creste, Camila Fernanda Zorzella ^{[1, 2]} ; Orsi, Patricia Rodrigues ^[1] ; Landim-Alvarenga, Fernanda Cruz ^[3] ; Justulin, Luis Antonio ^[4] ; Golim, Marjorie de Assis ^[2] ; Barraviera, Benedito ^{[1, 2]} ; Ferreira, Rui Seabra ^{[1, 2]}

Affiliation

^[1] UNESP Sao Paulo State Univ, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP - Brazil

^[2] UNESP Sao Paulo State Univ, Botucatu Med Sch, BR-18618687 Botucatu, SP - Brazil

^[3] UNESP Sao Paulo State Univ, Coll Vet Med & Anim Husb FMVZ, BR-18618681 Botucatu, SP - Brazil

^[4] UNESP Sao Paulo State Univ, Botucatu Biosci Inst, BR-18618689 Botucatu, SP - Brazil

• Document type: Journal article
• Source: MATERIALS; v. 13, n. 12 JUN 2020.
• Web of Science Citations: 0

Abstract

Fibrin scaffold fits as a provisional platform promoting cell migration and proliferation, angiogenesis, connective tissue formation and growth factors stimulation. We evaluated a unique heterologous fibrin biopolymer as scaffold to mesenchymal stem cells (MSCs) to treat a critical-size bone defect. Femurs of 27 rats were treated with fibrin biopolymer (FBP); FBP + MSCs; and FBP + MSC differentiated in bone lineage (MSC-D). Bone repair was evaluated 03, 21 and 42 days later by radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP + MSC-D association was the most effective treatment, since newly formed Bone was more abundant and early matured in just 21 days. We concluded that FBP is an excellent scaffold for MSCs and also use of differentiated cells should be encouraged in regenerative therapy researches. The FBP ability to maintain viable MSCs at Bone defect site has modified inflammatory environment and accelerating their regeneration. (AU)

• FAPESP's process: 13/02004-3 - Evaluation of snake venom-derived fibrin sealant as a biological scaffold for mesenchymal stem cells in rat femur bone regeneration
• Grantee: Camila Fernanda Zorzella Creste
• Support Opportunities: Scholarships in Brazil - Master