Use of fibrin sealant associated with hydroxyapatite in repair of bone defects induced in rat calvaria

• Grant number: 12/02689-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2012
• End date: April 30, 2013
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Marcelo Rodrigues da Cunha
• Grantee: Fernanda de Araujo Menezes
• Host Institution: Centro Universitário Padre Anchieta (UniAnchieta). Sociedade Padre Anchieta de Ensino (SPAE). Jundiaí , SP, Brazil

Abstract

Congenital deformities, trauma, and tumors with significant loss of bone mass can be repaired using autograft. However, their use may become limited due to some disadvantages, such as chronic pain and necrosis of the donor area. Thus, synthetic biomaterials are a viable alternative to grafting. Among these materials, stands out for its biocompatibility and hydroxyapatite composition similar to bone. Another substance with osteogenic potential is likely fibrin sealant due to its healing properties acting as an adhesive to the fabric and probable carrier for cell proliferation. Thus, this study aimed at assessing the process of bone formation during the repair of cranial bone defects grafted with fibrin sealant and hydroxyapatites. To this, 32 rats will be used. The animals will undergo the surgical procedure to create a critical bone defect in the skull to be filled with fibrin sealant and hydroxyapatite. 2 and 6 weeks after surgery, the animals will be sacrificed and samples of the receiving area of the implants will undergo radiological analyzes, histological and morphometric.(AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ORSI, PATRICIA RODRIGUES; LANDIM-ALVARENGA, FERNANDA CRUZ; JUSTULIN, JR., LUIS ANTONIO; KANENO, RAMON; GOLIM, MARJORIE DE ASSIS; DOS SANTOS, DANIELA CARVALHO; ZORZELLA CRESTE, CAMILA FERNANDA; OBA, EUNICE; MAIA, LEANDRO; BARRAVIERA, BENEDITO; et al. A unique heterologous fibrin sealant (HFS) as a candidate biological scaffold for mesenchymal stem cells in osteoporotic rats. STEM CELL RESEARCH & THERAPY, v. 8, SEP 29 2017. (14/06001-1, 09/53846-9, 12/02689-3, 12/08101-8)

BISCOLA, NATALIA PERUSSI; CARTAROZZI, LUCIANA POLITTI; ULIAN-BENITEZ, SUZANA; BARBIZAN, ROBERTA; CASTRO, MATEUS VIDIGAL; SPEJO, ALINE BARROSO; FERREIRA, JR., RUI SEABRA; BARRAVIERA, BENEDITO; RODRIGUES OLIVEIRA, ALEXANDRE LEITE. Multiple uses of fibrin sealant for nervous system treatment following injury and disease. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 1, MAR 14 2017. (12/20456-6, 12/08101-8, 14/06892-3, 12/22750-9, 12/02689-3, 11/23377-7, 11/02889-0, 13/04409-0, 09/53846-9, 14/11405-4, 10/00729-2, 13/23030-2, 12/13514-0)

DA CUNHA, MARCELO RODRIGUES; MENEZES, FERNANDA ARAUJO; DOS SANTOS, GEOVANE RIBEIRO; LOPES PINTO, CLOVIS ANTONIO; BARRAVIERA, BENEDITO; AMARO MARTINS, VIRGINIA DA CONCEICAO; DE GUZZI PLEPIS, ANA MARIA; FERREIRA JUNIOR, RUI SEABRA. Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 18, n. 1, p. 196-203, JAN-FEB 2015. (12/08101-8, 09/06280-0, 12/02689-3, 09/53846-9)

NATALIA PERUSSI BISCOLA; LUCIANA POLITTI CARTAROZZI; SUZANA ULIAN-BENITEZ; ROBERTA BARBIZAN; MATEUS VIDIGAL CASTRO; ALINE BARROSO SPEJO; RUI SEABRA FERREIRA JR.; BENEDITO BARRAVIERA; ALEXANDRE LEITE RODRIGUES OLIVEIRA. Multiple uses of fibrin sealant for nervous system treatment following injury and disease. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, 2017-04-27. (13/23030-2, 11/23377-7, 12/02689-3, 12/20456-6, 14/06892-3, 14/11405-4, 11/02889-0, 13/04409-0, 12/08101-8, 09/53846-9, 12/22750-9, 12/13514-0, 10/00729-2)

DA SILVA, D. A. F.; BISCOLA, N. P.; DOS SANTOS, L. D.; SARTORI, M. M. P.; DENADAI, J. C.; DA SILVA, E. T.; DUCATTI, C.; BICUDO, S. D.; BARRAVIERA, B.; FERREIRA, JR., R. S.. Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS). VETERINARY JOURNAL, v. 217, p. 119-125, NOV 2016. (09/53846-9, 12/02689-3, 12/08101-8)

DA CUNHA, MARCELO RODRIGUES; MENEZES, FERNANDA ARAUJO; DOS SANTOS, GEOVANE RIBEIRO; LOPES PINTO, CLOVIS ANTONIO; BARRAVIERA, BENEDITO; AMARO MARTINS, VIRGINIA DA CONCEICAO; DE GUZZI PLEPIS, ANA MARIA; FERREIRA JUNIOR, RUI SEABRA. Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 18, n. 1, p. 8-pg., 2015-01-01. (12/02689-3, 12/08101-8, 09/06280-0, 09/53846-9)