Multiple uses of fibrin sealant for nervous system treatment following injury and disease

Author(s)

Biscola, Natalia Perussi ; Cartarozzi, Luciana Politti ; Ulian-Benitez, Suzana ; Barbizan, Roberta ; Castro, Mateus Vidigal ; Spejo, Aline Barroso ; Ferreira, Jr., Rui Seabra ; Barraviera, Benedito ; Rodrigues Oliveira, Alexandre Leite

• Document type: Review article
• Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 23, n. 1 MAR 14 2017.
• Web of Science Citations: 19

Abstract

Lesions to the nervous system often produce hemorrhage and tissue loss that are difficult, if not impossible, to repair. Therefore, scar formation, inflammation and cavitation take place, expanding the lesion epicenter. This significantly worsens the patient conditions and impairment, increasing neuronal loss and glial reaction, which in turn further decreases the chances of a positive outcome. The possibility of using hemostatic substances that also function as a scaffold, such as the fibrin sealant, reduces surgical time and improve postoperative recovery. To date, several studies have demonstrated that human blood derived fibrin sealant produces positive effects in different interventions, becoming an efficient alternative to suturing. To provide an alternative to homologous fibrin sealants, the Center for the Study of Venoms and Venomous Animals (CEVAP, Brazil) has proposed a new bioproduct composed of certified animal components, including a thrombin-like enzyme obtained from snake venom and bubaline fibrinogen. Thus, the present review brings up to date literature assessment on the use of fibrin sealant for nervous system repair and positions the new heterologous bioproduct from CEVAP as an alternative to the commercial counterparts. In this way, clinical and pre-clinical data are discussed in different topics, ranging from central nervous system to peripheral nervous system applications, specifying positive results as well as future enhancements that are necessary for improving the use of fibrin sealant therapy. (AU)

• FAPESP's process: 12/20456-6 - The involvement of the major histocompatibility complex class I molecules in the astrocytic reactivity after sciatic nerve transection
• Grantee: Sérgio Luiz Oliveira Nunes
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/08101-8 - In vivo imaging of nerve regeneration following ventral root replantation with fluo-stained fibrin sealant associated with mesenchymal stem cells
• Grantee: Rui Seabra Ferreira Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/06892-3 - Use of mesenchymal stem cells in the CNS/PNS interface: repair of proximal lesions
• Grantee: Alexandre Leite Rodrigues de Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/22750-9 - Use of fibrin sealant combined with mesenchymal stem cells for anterior funiculus injury repair: effects on inflammation, axonal regeneration and neuroprotection.
• Grantee: Aline Barroso Spejo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/02689-3 - Use of fibrin sealant associated with hydroxyapatite in repair of bone defects induced in rat calvaria
• Grantee: Fernanda de Araujo Menezes
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 11/23377-7 - Neuroprotective and regenerative effects of fibrin sealant derived from snake venom combined with mononuclear stem cells after neurorrhaphy of the sciatic nerve of neonatal rats
• Grantee: Natália Perussi Biscola
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 11/02889-0 - Use of fibrin sealant combined with mesenchymal stem cells in the repair of peripheral nerves through tubulization technique
• Grantee: Luciana Politti Cartarozzi
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/04409-0 - Comparative study on the use of commercial and snake venon derived fibrin sealant for reimplantation of spinal cord ventral roots following avulsion
• Grantee: Mateus Vidigal de Castro
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/53846-9 - Recent acquisition of preparative high-performance liquid chromatograph for the purification and isolation of serine proteinases to produce fibrin sealant
• Grantee: Benedito Barraviera
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 14/11405-4 - Alpha/gamma motoneuron survival rate and plasticity following neonatal sciatic nerve axotomy
• Grantee: Natália Perussi Biscola
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• FAPESP's process: 10/00729-2 - The use of a fibrin sealant combined with bonemarrow mononuclear stem cells to repair motor roots after avulsion in the interface of CNS/PNS
• Grantee: Roberta Barbizan Petinari
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/23030-2 - Application of human embryonic stem cells overexpressing FGF2 after ventral root avulsion: neuroprotection and immunomodulation
• Grantee: Marta Rocha Araujo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/13514-0 - Analysis of cellular mechanisms of acute injuries in the central and peripheral nervous system using novel imaging approaches
• Grantee: Luciana Politti Cartarozzi
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree