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Comparative study on the use of commercial and snake venon derived fibrin sealant for reimplantation of spinal cord ventral roots following avulsion

Grant number: 13/04409-0
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2013
Effective date (End): June 30, 2015
Field of knowledge:Biological Sciences - Morphology - Cytology and Cell Biology
Principal researcher:Alexandre Leite Rodrigues de Oliveira
Grantee:Mateus Vidigal de Castro
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Ventral root avulsion (VRA) is an experimental model of proximal axonal injury at the central/peripheral nervous system interface that results in significant neuronal death. In order to decrease neuronal degeneration and treating such injuries, the reimplantation of avulsed roots is an alternative approach. Such procedure may be done by direct suturing the avulsed roots to the surface of the spinal cord or by the use of fibrin sealant. The aim of the present work is to investigate whether snake venom derived fibrin glue is as effective as the commercial counterpart. In this sense, female Lewis rats (7 weeks old) will be subjected to VRA and roots reimplantation. The animals will be divided into the following groups (n=10 for each group): Group 1 - avulsion only; Group 2 - avulsion followed by root repair by using commercial fibrin glue (Beriplast® P); Group 3- root reimplantation with fibrin sealant derived from snake venom. The animals will be monitored up to 4 and 12 weeks post-surgery and will be sacrificed. Their lumbar intumescences and sciatic nerves will be processed for motoneuron counting, regeneratied axons morphometry and immunohistochemistry (antisera to be used: anti-GFAP- glial fibrillary acidic protein, an astroglial marker; Iba-1- ionized calcium binding adaptor molecule, a microglial marker; synaptophysin - a synaptic marker, Chat - acetyl choline transferase, p75NTR - pan neurotrophin receptor, S100 - Schwann cell marker and laminin). Motor recovery will be evaluated with the walking track test, by using the CatWalk system (Noldus Inc.) from the time of the lesion up to 12 weeks post root repair.

News published in Agência FAPESP Newsletter about the scholarship:

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BISCOLA, NATALIA PERUSSI; CARTAROZZI, LUCIANA POLITTI; ULIAN-BENITEZ, SUZANA; BARBIZAN, ROBERTA; CASTRO, MATEUS VIDIGAL; SPEJO, ALINE BARROSO; FERREIRA, JR., RUI SEABRA; BARRAVIERA, BENEDITO; RODRIGUES OLIVEIRA, ALEXANDRE LEITE. Multiple uses of fibrin sealant for nervous system treatment following injury and disease. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 1 MAR 14 2017. Web of Science Citations: 19.
NATALIA PERUSSI BISCOLA; LUCIANA POLITTI CARTAROZZI; SUZANA ULIAN-BENITEZ; ROBERTA BARBIZAN; MATEUS VIDIGAL CASTRO; ALINE BARROSO SPEJO; RUI SEABRA FERREIRA JR.; BENEDITO BARRAVIERA; ALEXANDRE LEITE RODRIGUES OLIVEIRA. Multiple uses of fibrin sealant for nervous system treatment following injury and disease. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, p. -, 2017.
DE CASTRO, MATEUS VIDIGAL; BARBIZAN, ROBERTA; FERREIRA, JR., RUI SEABRA; BARRAVIERA, BENEDITO; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE. Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration. NEURAL PLASTICITY, 2016. Web of Science Citations: 4.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CASTRO, Mateus Vidigal de. Comparative study on the use of commercial and snake venon derived fibrin sealant for reimplantation of spinal cord ventral roots following avulsion. 2015. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.