Alpha/gamma motoneuron survival rate and plasticity following neonatal sciatic nerve axotomy

• Grant number: 14/11405-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: October 15, 2014
• End date: August 14, 2015
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Benedito Barraviera
• Grantee: Natália Perussi Biscola
• Supervisor: Leif A. Havton
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: University of California, Irvine (UC Irvine), United States
• Associated to the scholarship: 11/23377-7 - Neuroprotective and regenerative effects of fibrin sealant derived from snake venom combined with mononuclear stem cells after neurorrhaphy of the sciatic nerve of neonatal rats, BP.DD

Abstract

The nervous system of neonatal rats is more sensitive to injury, so that peripheral nerve axotomy leads to degeneration of spinal motor and sensory neurons. Similarly, crushing of sciatic nerve results in significant loss of motoneurons. One important question is whether there is a preferential alpha or gamma motoneuron degeneration, or even plasticity of the surviving neurons, to innervate extrafusal muscle fibers. If so, the investigation of potential alpha/gamma differentiation is of great interest. Thus, the aims of the present study are to investigate potential alpha/gamma compensatory plasticity and to quantify surviving motoneurons after fluorogold labeling. The animals will be divided into two groups: GC: Control Group- non-operated and GS: sciatic nerve crushing. The pups will be lesioned at P2 and evaluated following 2 and 4 weeks by transmission electron microscopy, for synaptic plasticity analysis; and immunohistochemistry and light microscopy for neuronal survival. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BISCOLA, NATALIA PERUSSI; CARTAROZZI, LUCIANA POLITTI; ULIAN-BENITEZ, SUZANA; BARBIZAN, ROBERTA; CASTRO, MATEUS VIDIGAL; SPEJO, ALINE BARROSO; FERREIRA, JR., RUI SEABRA; BARRAVIERA, BENEDITO; RODRIGUES OLIVEIRA, ALEXANDRE LEITE. Multiple uses of fibrin sealant for nervous system treatment following injury and disease. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 1, MAR 14 2017. (12/20456-6, 12/08101-8, 14/06892-3, 12/22750-9, 12/02689-3, 11/23377-7, 11/02889-0, 13/04409-0, 09/53846-9, 14/11405-4, 10/00729-2, 13/23030-2, 12/13514-0)

NATALIA PERUSSI BISCOLA; LUCIANA POLITTI CARTAROZZI; SUZANA ULIAN-BENITEZ; ROBERTA BARBIZAN; MATEUS VIDIGAL CASTRO; ALINE BARROSO SPEJO; RUI SEABRA FERREIRA JR.; BENEDITO BARRAVIERA; ALEXANDRE LEITE RODRIGUES OLIVEIRA. Multiple uses of fibrin sealant for nervous system treatment following injury and disease. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, 2017-04-27. (13/23030-2, 11/23377-7, 12/02689-3, 12/20456-6, 14/06892-3, 14/11405-4, 11/02889-0, 13/04409-0, 12/08101-8, 09/53846-9, 12/22750-9, 12/13514-0, 10/00729-2)