Development and biological evaluation of a new calcium phosphate with and without fibrin sealant: an in vivo study in rabbits and in vitro study

• Grant number: 14/16688-4
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): November 01, 2014
• Effective date (End): June 29, 2017
• Field of knowledge: Health Sciences - Dentistry
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Joni Augusto Cirelli
• Grantee: Fernando Pozzi Semeghini Guastaldi
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 17/06137-9 - 3D printing, modifying current micro architecture to increase neovascularization in the center and in vitro assessment, BE.EP.PD

Abstract

Purpose: The aim of this study will be to develop and evaluate a new calcium phosphate with and without fibrin sealant, in bone regeneration of critical size defects in rabbit calvarias. Materials and Methods: Forty-eight (New Zealand) rabbits will be used. Bilateral critical size defects (10-mm diameter) will be created in the parietal bones. Six groups of 8 animals each evaluated: Group I (experimental calcium phosphate), Group II (experimental calcium phosphate + fibrin sealant), Group III (coagulum), Group IV (autogenous bone), Group V (Bio-Oss) and Group V (Cerasorb). All defects will be covered by a slow resorbing collagen membrane (Bio-Guide). The animals will be euthanized at 1, 2, 4 and 8 weeks. Analysis: Prior to surgery the biomaterials used in this study will be submitted to physico-chemical and morphological characterization, using the following analysis: Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) and Infrared (IR). Subsequently the surgical procedure in the animals and respecting the periods of the proposed experimental groups, the following analyses will be performed: histological, histometric, immunohistochemical and micro-CT. Regarding the in vitro analysis, proliferation assays and cell viability, Elisa, RT-PCR, SEM, alkaline phosphatase activity and mineralization nodule formation will be performed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTANA, LUIS CARLOS L.; GUASTALDI, FERNANDO POZZI S.; IDOGAVA, HENRIQUE T.; NORITOMI, PEDRO Y.; DE FOGGI, CAMILA C.; VAZ, LUIS G.. Mechanical Stress Analysis of Different Configurations of the All-on-4 Concept in Atrophic Mandible: A 3D Finite Element Study. INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS, v. 36, n. 1, p. 75-85, JAN-FEB 2021. (14/16688-4)

SEMEGHINI GUASTALDI, FERNANDO POZZI; DE FOGGI, CAMILA CRISTINA; LEAL SANTANA, LUIS CARLOS; VAZ, LUIS GERALDO; VERGANI, CARLOS EDUARDO; GUASTALDI, ANTONIO CARLOS. Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 22, n. 4, 2019. (14/16688-4)