Bacterial Abilities and Adaptation Toward the Rhizosphere Colonization

Autor(es)

Lopes, Lucas D. ; Pereira e Silva, Michele de Cassia ; Andreote, Fernando D.

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 7, AUG 25 2016.
• Citações Web of Science: 19

Resumo

The rhizosphere harbors one of the most complex, diverse, and active plant -associated microbial communities. This community can be recruited by the plant host to either supply it with nutrients or to help in the survival under stressful conditions. Although selection for the rhizosphere community is evident, the specific bacterial traits that make them able to colonize this environment are still poorly understood. Thus, here we used a combination of community level physiological profile (CLPP) analysis and 16S rRNA gene quantification and sequencing (coupled with in silico analysis and metagenome prediction), to get insights on bacterial features and processes involved in rhizosphere colonization of sugarcane. CLPP revealed a higher metabolic activity in the rhizosphere compared to bulk soil, and suggested that D-galacturonic acid plays a role in bacterial selection by the plant roots (supported by results of metagenome prediction). Quantification of the 16S rRNA gene confirmed the higher abundance of bacteria in the rhizosphere. Sequence analysis showed that of the 252 classified families sampled, 24 were significantly more abundant in the bulk soil and 29 were more abundant in the rhizosphere. Furthermore, metagenomes predicted from the 16S rRNA gene sequences revealed a significant higher abundance of predicted genes associated with biofilm formation and with horizontal gene transfer (HGT) processes. In sum, this study identified major bacterial groups and their potential abilities to occupy the sugarcane rhizosphere, and indicated that polygalacturonase activity and HGT events may be important features for rhizosphere colonization. (AU)

• Processo FAPESP: 13/50353-7 - Microbial consortia for biowaste management: life cycle analysis of novel strategies of bioconversion (MICROWASTE)
• Beneficiário: Fernando Dini Andreote
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular