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GBS-based single dosage markers for linkage and QTL mapping allow gene mining for yield-related traits in sugarcane

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Almeida Balsalobre, Thiago Willian ; Pereira, Guilherme da Silva ; Alves Margarido, Gabriel Rodrigues ; Gazaffi, Rodrigo ; Barreto, Fernanda Zatti ; Anoni, Carina Oliveira ; Cardoso-Silva, Claudio Benicio ; Costa, Estela Araujo ; Mancini, Melina Cristina ; Hoffmann, Hermann Paulo ; de Souza, Anete Pereira ; Franco Garcia, Antonio Augusto ; Carneiro, Monalisa Sampaio
Número total de Autores: 13
Tipo de documento: Artigo Científico
Fonte: BMC Genomics; v. 18, JAN 11 2017.
Citações Web of Science: 22

Background: Sugarcane (Saccharum spp.) is predominantly an autopolyploid plant with a variable ploidy level, frequent aneuploidy and a large genome that hampers investigation of its organization. Genetic architecture studies are important for identifying genomic regions associated with traits of interest. However, due to the genetic complexity of sugarcane, the practical applications of genomic tools have been notably delayed in this crop, in contrast to other crops that have already advanced to marker-assisted selection (MAS) and genomic selection. High-throughput next-generation sequencing (NGS) technologies have opened new opportunities for discovering molecular markers, especially single nucleotide polymorphisms (SNPs) and insertion-deletion (indels), at the genome-wide level. The objectives of this study were to (i) establish a pipeline for identifying variants from genotyping-by-sequencing (GBS) data in sugarcane, (ii) construct an integrated genetic map with GBS-based markers plus target region amplification polymorphisms and microsatellites, (iii) detect QTLs related to yield component traits, and (iv) perform annotation of the sequences that originated the associated markers with mapped QTLs to search putative candidate genes. Results: We used four pseudo-references to align the GBS reads. Depending on the reference, from 3,433 to 15,906 high-quality markers were discovered, and half of them segregated as single-dose markers (SDMs) on average. In addition to 7,049 non-redundant SDMs from GBS, 629 gel-based markers were used in a subsequent linkage analysis. Of 7,678 SDMs, 993 were mapped. These markers were distributed throughout 223 linkage groups, which were clustered in 18 homo(eo)logous groups (HGs), with a cumulative map length of 3,682.04 cM and an average marker density of 3.70 cM. We performed QTL mapping of four traits and found seven QTLs. Our results suggest the presence of a stable QTL across locations. Furthermore, QTLs to soluble solid content (BRIX) and fiber content (FIB) traits had markers linked to putative candidate genes. Conclusions: This study is the first to report the use of GBS for large-scale variant discovery and genotyping of a mapping population in sugarcane, providing several insights regarding the use of NGS data in a polyploid, non-model species. The use of GBS generated a large number of markers and still enabled ploidy and allelic dosage estimation. Moreover, we were able to identify seven QTLs, two of which had great potential for validation and future use for molecular breeding in sugarcane. (AU)

Processo FAPESP: 10/50549-0 - Construção de um mapa funcional e mapeamento FR QTLs de importância econômica em uma população derivada de cruzamento comercial bi-parental em cana-de-açúcar
Beneficiário:Melina Cristina Mancini
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/25236-4 - Mapeamento comparativo de QTLs e sintenia genômica entre sorgo sacarino e cana-de-açúcar
Beneficiário:Guilherme da Silva Pereira
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 10/50031-1 - Mapeamento genético-funcional em cana-de-açúcar e localização de QTLs de importância econômica
Beneficiário:Estela Araujo Costa
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 10/50091-4 - Mapeamento de QTLs em população derivada de cruzamento comercial bi-parental em cana-de-açúcar
Beneficiário:Thiago Willian Almeida Balsalobre
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 08/52197-4 - Genomic-assisted breeding of sugarcane: using molecular markers for understanding the genetic architecture of quantitative traits and to implement marker assisted selection
Beneficiário:Anete Pereira de Souza
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 12/11109-0 - Análise in silico do transcriptoma e de sequências genômicas de cana-de-açúcar: identificação de SNPs, análise da expressão alelo-específica e desenvolvimento e caracterização de microssatélites
Beneficiário:Cláudio Benício Cardoso da Silva
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
Beneficiário:Marcos Silveira Buckeridge
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático