Ru(II)/clotrimazole/diphenylphosphine/bipyridine complexes: Interaction with DNA, BSA and biological potential against tumor cell lines and Mycobacterium tuberculosis

Autor(es)

Colina-Vegas, Legna ; Dutra, Jocely Lucena ; Villarreal, Wilmer ; Neto, Joao Honorato de A. ; Cominetti, Marcia Regina ; Pavan, Fernando ; Navarro, Maribel ; Batista, Alzir A.

• Tipo de documento: Artigo Científico
• Fonte: Journal of Inorganic Biochemistry; v. 162, p. 135-145, SEP 2016.
• Citações Web of Science: 16

Resumo

Three ruthenium complexes {[}RuCl(CTZ)(bipy)(P-P)]PF6 {[}P-P = 1,2-bis(diphenylphosphino)ethane (dppe-1), 1,4-bis(diphenylphosphino)butane (dppb-2) and 1,1'-bis(diphenylphosphino)ferrocene (dppf-3), bipy = 2,2'-bipiridine and clotrimazole (CTZ) 1-{[}(2-chlorophenyl)diphenylmethyl]-1H-imidazole] were synthesized. These complexes were characterized by a combination of elemental analysis, molar conductivity, infrared and UV-vis spectroscopy, H-1, C-13[H-1] and P-31[H-1] nuclear magnetic resonance techniques, cyclic voltammetry and mass spectroscopy. Bovine serum albumin binding constants, which were in the range of 130-36.00 x 10(4) M-1, and thermodynamic parameters suggest spontaneous interactions with this protein by electrostatic forces due to the positive charge of the complexes. DNA interactions studied by spectroscopic titration, viscosity measurements, gel electrophoresis, circular dichroism, ethidium bromide displacement and reactions with guanosine and guanosine monophosphate indicated the DNA binding affinity primarily through non-covalent interactions. All complexes 1-3 were tested against the human carcinoma cell lines MCF-7 (breast), A549 (lung) and DU-145 (prostate) presenting promising IC50 values, between 0.50 and 14.00 mu M, in some cases lower than the IC50 for the reference drug (cisplatin). The antimicrobial activity assays of the complexes provided evidence that they are potential agents against mycobacterial infections, specifically against Mycobacterium tuberculosis H37Rv. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 13/03513-9 - Sínteses e caracterização de complexos [RuCl(bipy)(P-P)CTZ]PF6 (P-P= dppe, dppb e dppf ; CTZ= clotrimazol) e avaliação da interação com o DNA
• Beneficiário: Jocely de Lucena Dutra
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica