Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production

Autor(es)

Castro-Alves, Victor Costa ; Gomes, Daniel ; Menolli, Jr., Nelson ; Sforca, Mauricio Luis ; Oliveira do Nascimento, Joao Roberto

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 95, p. 215-223, FEB 2017.
• Citações Web of Science: 13

Resumo

Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects. In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo-(MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages. Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as beta-(1,6)-branched beta-(1,3)-glucans, BaHW and MyEN as alpha-(1,3)-(1,2)-branched alpha-(1,6)-glucans, and BaHA as a mixture of alpha-(1,6) and beta-(1,3)-glucans. BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-alpha) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles. In contrast, BaHW and MyEN induced TNF-alpha, NO and IL-6 production, and increased zymosan phagocytosis, while BaHA displayed intermediary effects in comparison the other polysaccharides. In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable alpha- and beta-glucans with potential immunomodulatory effects. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 16/05083-0 - Efeito de polissacarídeos de origem fúngica e vegetal do tipo não-amido sobre a função de macrófagos
• Beneficiário: Victor Costa Castro Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs