Cytotoxicity and Bioactivity of Calcium Silicate Cements Combined with Niobium Oxide in Different Cell Lines

Autor(es)

Leticia Boldrin Mestieri ^[1] ; Ana Lívia Gomes-Cornélio ^[2] ; Elisandra Márcia Rodrigues ^[3] ; Gisele Faria ^[4] ; Juliane Maria Guerreiro-Tanomaru ^[5] ; Mário Tanomaru-Filho ^[6]

Afiliação do(s) autor(es)

^[1] Universidade Estadual Paulista - Brasil

^[2] Universidade Estadual Paulista - Brasil

^[3] Universidade Estadual Paulista - Brasil

^[4] Universidade Estadual Paulista - Brasil

^[5] Universidade Estadual Paulista - Brasil

^[6] Universidade Estadual Paulista - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 28, n. 1, p. 65-71, 2017-02-00.

Resumo

Abstract The aim of this study was to evaluate the cytotoxicity and bioactivity of calcium silicate-based cements combined with niobium oxide (Nb2O5) micro and nanoparticles, comparing the response in different cell lines. This evaluation used four cell lines: two primary cultures (human dental pulp cells - hDPCs and human dental follicle cells - hDFCs) and two immortalized cultures (human osteoblast-like cells - Saos-2 and mouse periodontal ligament cells - mPDL). The tested materials were: White Portland Cement (PC), mineral trioxide aggregate (MTA), white Portland cement combined with microparticles (PC/Nb2O5µ) or nanoparticles (PC/Nb2O5n) of niobium oxide (Nb2O5). Cytotoxicity was evaluated by the methylthiazolyldiphenyl-tetrazolium bromide (MTT) and trypan blue exclusion assays and bioactivity by alkaline phosphatase (ALP) enzyme activity. Results were analyzed by ANOVA and Tukey test (a=0.05). PC/Nb2O5n presented similar or higher cell viability than PC/Nb2O5µ in all cell lines. Moreover, the materials presented similar or higher cell viability than MTA. Saos-2 exhibited high ALP activity, highlighting PC/Nb2O5µ material at 7 days of exposure. In conclusion, calcium silicate cements combined with micro and nanoparticles of Nb2O5 presented cytocompatibility and bioactivity, demonstrating the potential of Nb2O5 as an alternative radiopacifier agent for these cements. The different cell lines had similar response to cytotoxicity evaluation of calcium silicate cements. However, bioactivity was more accurately detected in human osteoblast-like cell line, Saos-2. (AU)

• Processo FAPESP: 12/08961-7 - Viabilidade e bioatividade celular de materiais à base de Cimento Portland em cultura primária de células do folículo e polpa dental humanos, fibroblastos e osteoblastos
• Beneficiário: Leticia Boldrin Mestieri
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/13268-9 - Viabilidade e bioatividade celular de materiais à base de cimento Portland em cultura primária de células do folículo e polpa dental humanos, fibroblastos e osteoblastos
• Beneficiário: Mario Tanomaru Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular