Could mucin 16 and colony-stimulating factor 2-receptor beta possible graft versus host disease biomarkers? Medical hypotheses

Autor(es)

de Souza, Milena Monteiro ; de Paula, Fabiana Martins ; Hsieh, Ricardo ; Martins Almeida Macedo, Maria Cristina ; Corral, Marcelo Andreetta ; Nunes, Thais Borguezan ; De Paula, Fernanda ; Lourenco, Silvia Vanessa

• Tipo de documento: Artigo Científico
• Fonte: Medical Hypotheses; v. 100, p. 89-93, MAR 2017.
• Citações Web of Science: 2

Resumo

Graft versus host disease (GVHD) occurs after bone marrow transplantation and is one of the most important causes of death worldwide. Reviews demonstrated GVHD patients with involvement of various tissues and organs, such as salivary glands. The diagnosis of acute GVHD has been the biopsies and the histopathologic evaluation of tissue from an involved organ. These procedures are invasive. Saliva proteins as possible biomarker for GVHD could facilitate the management and diagnosis accuracy. For support the proposed hypotheses, in this pilot study we collected whole saliva samples from patients with undergoing allogeneic hematopoietic cell transplantation (HCT) and from healthy subjects. Samples were collected prospectively between pre-transplant, thirty days, one hundred and, two hundred days after transplant. The proteomic profile was analyzed using SDS-PAGE and LCMS-ESI-IT-TOF mass spectrometry. The relevant personal data, past medical history were also recorded. The most relevant proteins found exclusively in GVHD patients were: CSF2RB, Protocadherin (Pcdh) Fat 2 precursor, protein capicua homolog isoform CIC-S, MUC16 and RGPD8\_HUMAN RANBP2. This study aims to conduct an initial evaluation of the possible presence of such biomarkers in saliva from GVHD patients, and suggested a potential application of proteomics analysis as a alternative method to diagnose GVHD. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/11020-5 - O ENVOLVIMENTO DAS GLÂNDULAS SALIVARES NA DOENÇA DO ENXERTO CONTRA O HOSPEDEIRO: Estudo bioquímico e análise proteômica.
• Beneficiário: Silvia Vanessa Lourenço
• Modalidade de apoio: Auxílio à Pesquisa - Regular