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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Could mucin 16 and colony-stimulating factor 2-receptor beta possible graft versus host disease biomarkers? Medical hypotheses

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Author(s):
de Souza, Milena Monteiro ; de Paula, Fabiana Martins ; Hsieh, Ricardo ; Martins Almeida Macedo, Maria Cristina ; Corral, Marcelo Andreetta ; Nunes, Thais Borguezan ; De Paula, Fernanda ; Lourenco, Silvia Vanessa
Total Authors: 8
Document type: Journal article
Source: Medical Hypotheses; v. 100, p. 89-93, MAR 2017.
Web of Science Citations: 2
Abstract

Graft versus host disease (GVHD) occurs after bone marrow transplantation and is one of the most important causes of death worldwide. Reviews demonstrated GVHD patients with involvement of various tissues and organs, such as salivary glands. The diagnosis of acute GVHD has been the biopsies and the histopathologic evaluation of tissue from an involved organ. These procedures are invasive. Saliva proteins as possible biomarker for GVHD could facilitate the management and diagnosis accuracy. For support the proposed hypotheses, in this pilot study we collected whole saliva samples from patients with undergoing allogeneic hematopoietic cell transplantation (HCT) and from healthy subjects. Samples were collected prospectively between pre-transplant, thirty days, one hundred and, two hundred days after transplant. The proteomic profile was analyzed using SDS-PAGE and LCMS-ESI-IT-TOF mass spectrometry. The relevant personal data, past medical history were also recorded. The most relevant proteins found exclusively in GVHD patients were: CSF2RB, Protocadherin (Pcdh) Fat 2 precursor, protein capicua homolog isoform CIC-S, MUC16 and RGPD8\_HUMAN RANBP2. This study aims to conduct an initial evaluation of the possible presence of such biomarkers in saliva from GVHD patients, and suggested a potential application of proteomics analysis as a alternative method to diagnose GVHD. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 14/11020-5 - SALIVARY GLANDS INVOLVEMENT IN GRAFT VERSUS HOST DISEASE: Biochemical and proteomic analysis.
Grantee:Silvia Vanessa Lourenço
Support Opportunities: Regular Research Grants