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Molecular and antimicrobial susceptibility characterization of Globicatella sulfidifaciens isolated from sow's urinary tract infection

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Matajira, Carlos E. C. ; Moreno, Luisa Z. ; Gomes, Vasco T. M. ; Silva, Ana Paula S. ; Mesquita, Renan E. ; Amigo, Cristina R. ; Christ, Ana Paula G. ; Sato, Maria Ines Z. ; Moreno, Andrea M.
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: VETERINARY QUARTERLY; v. 37, n. 1, p. 48-51, 2017.
Citações Web of Science: 0

Background: The Globicatella genus comprises Gram-positive, facultative anaerobic, a-hemolytic and catalase negative cocci morphologically and phenotypically very similar to Streptococcus and Aerococcus genus which can lead to misidentification and underestimation of this pathogen. Globicatella species have already been isolated from human and animals with heart and brain disorders. Their clinical relevance in animals, and its zoonotic potential, remains unknown due to the difficulty in their identification. Objective: To present the isolation, phenotypic and molecular characterization of G. sulfidifaciens from urinary tract infection in sows. Materials and Methods: Urine samples from 140 sows of two swine herds located in Sao Paulo State (Brazil) yielded the isolation of three presumptive G. sulfidifaciens strains. Identification and species confirmation were done by MALDI-TOF MS and 16S rRNA sequencing. Strains were further characterized by single enzyme amplified fragments length polymorphism (SE-AFLP) and broth microdilution techniques. Results: All three isolates were confirmed as G. sulfidifaciens. The SE-AFLP genotyping resulted in distinct fingerprint patterns for each strain. All isolates presented high MIC values to tetracycline, sulphonamides, aminoglycosides and tylosin tartrate, which present high usage in human and animal medicine. Conclusions: Globicatella sulfidifaciens could be related to sporadic urinary tract infections in swine and appear to present alarming antimicrobial susceptibility profile. It is necessary to differentiate Streptococcus-like microorganisms in routine laboratory diagnostics for the correct identification of underestimated species potentially pathogenic to animals. (AU)

Processo FAPESP: 13/17136-2 - Caracterização de estirpes de Leptospira isoladas no Brasil através de tipificação por eletroforese em campo pulsado (PFGE) e análise comparativa de genomas
Beneficiário:Luisa Zanolli Moreno
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 15/26159-1 - Análise comparativa de genomas de estirpes de Streptococcus suis isoladas de suínos no Brasil
Beneficiário:Carlos Emilio Cabrera Matajira
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/16946-0 - Caracterização fenotipica e genotípica de cepas de Salmonella enterica isoladas de carne suína no Município de São Paulo
Beneficiário:Vasco Túlio de Moura Gomes
Linha de fomento: Bolsas no Brasil - Doutorado