Molecular and antimicrobial susceptibility characterization of Globicatella sulfidifaciens isolated from sow's urinary tract infection

Author(s)

Matajira, Carlos E. C. ; Moreno, Luisa Z. ; Gomes, Vasco T. M. ; Silva, Ana Paula S. ; Mesquita, Renan E. ; Amigo, Cristina R. ; Christ, Ana Paula G. ; Sato, Maria Ines Z. ; Moreno, Andrea M.

• Document type: Journal article
• Source: VETERINARY QUARTERLY; v. 37, n. 1, p. 48-51, 2017.
• Web of Science Citations: 0

Abstract

Background: The Globicatella genus comprises Gram-positive, facultative anaerobic, a-hemolytic and catalase negative cocci morphologically and phenotypically very similar to Streptococcus and Aerococcus genus which can lead to misidentification and underestimation of this pathogen. Globicatella species have already been isolated from human and animals with heart and brain disorders. Their clinical relevance in animals, and its zoonotic potential, remains unknown due to the difficulty in their identification. Objective: To present the isolation, phenotypic and molecular characterization of G. sulfidifaciens from urinary tract infection in sows. Materials and Methods: Urine samples from 140 sows of two swine herds located in Sao Paulo State (Brazil) yielded the isolation of three presumptive G. sulfidifaciens strains. Identification and species confirmation were done by MALDI-TOF MS and 16S rRNA sequencing. Strains were further characterized by single enzyme amplified fragments length polymorphism (SE-AFLP) and broth microdilution techniques. Results: All three isolates were confirmed as G. sulfidifaciens. The SE-AFLP genotyping resulted in distinct fingerprint patterns for each strain. All isolates presented high MIC values to tetracycline, sulphonamides, aminoglycosides and tylosin tartrate, which present high usage in human and animal medicine. Conclusions: Globicatella sulfidifaciens could be related to sporadic urinary tract infections in swine and appear to present alarming antimicrobial susceptibility profile. It is necessary to differentiate Streptococcus-like microorganisms in routine laboratory diagnostics for the correct identification of underestimated species potentially pathogenic to animals. (AU)

• FAPESP's process: 13/17136-2 - Characterization of Leptospira strains isolated in Brazil by pulsed field gel electrophoresis (PFGE) typing and comparative genomic analysis.
• Grantee: Luisa Zanolli Moreno
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/16946-0 - Phenotypic and genotypic characterization of strains of Salmonella enterica isolated from pork in São Paulo.
• Grantee: Vasco Túlio de Moura Gomes
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/26159-1 - Comparative genome analysis of Streptococcus suis strains isolated from swine in Brazil
• Grantee: Carlos Emilio Cabrera Matajira
• Support Opportunities: Scholarships in Brazil - Doctorate