Virulence assessment and phenotypic and genomic characterization of Corynebacterium spp. isolated from sow's urinary tract infection

• Grant number: 16/25745-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2017
• End date: January 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Andrea Micke Moreno
• Grantee: Luisa Zanolli Moreno
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The Corynebacterium genus still is poorly studied, despite the importance of toxigenic species for both human and animal health. The impact of the infection by these microorganisms on swine production has not yet been addressed, both in the international scenario and in the Brazilian reality, although pigs have been cited as carriers of Corynebacterium species. The aim of the present study is to characterize strains of Corynebacterium spp. isolated from sow's genito-urinary tract. The strains were previously identified as C. confusum, C. amycolatum, C. diphtheriae and for a group of six strains it was not possible to identify the species by MALDI-TOF MS and 16S rRNA and rpoB sequencing. Therefore, the present project proposes the assessment of the virulence of the different Corynebacterium species isolated from swine with the in vivo model of Galleria mellonella; the characterization and comparison of the respective biochemical profiles by the Biolog® system, and sequencing and comparative genomic analysis. The association of the in vivo virulence assessment with the genomic analysis will allow the identification of the genetic mechanisms of the observed virulence. The genomic comparison of strains of the same species, originated from different hosts, will also enable the identification of possible genetic predisposition to hosts. In addition, the genomic analysis will conclude the bacterial identification to confirm the suspected isolation of a new species or subspecies of Corynebacterium from swine in Brazil. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMIGO, CRISTINA ROMAN; MORENO, LUISA ZANOLLI; PORFIDA FERREIRA, THAIS SEBASTIANA; DE MOURA GOMES, VASCO TULIO; POOR, ANDRE PEGORARO; DE OLIVEIRA, CAROLINA HELENA; PARRA, BEATRIZ MARTINS; SANTOS SILVA, ANA PAULA; MORENO, ANDREA MICKE. Phenotypic, molecular and genomic characterization of Actinobaculum suis isolated from swine in Brazil. Anaerobe, v. 56, p. 27-33, APR 2019. (17/09515-4, 16/25745-7)

MORENO, LUISA Z.; COMES, VASCO T. M.; MOREIRA, JESSICA; DE OLIVEIRA, CAROLINA H.; PERES, BARBARA P.; SILVA, ANA PAULA S.; THAKUR, SIDDHARTHA; LA RAGIONE, ROBERTO M.; MORENO, ANDREA M.. First report of mcr-1-harboring Salmonella enterica serovar Schwarzengrund isolated from poultry meat in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 93, n. 4, p. 376-379, APR 2019. (16/25745-7, 13/16946-0)

CARDOSO, PEDRO H. M.; MORENO, LUISA Z.; DE OLIVEIRA, CAROLINA H.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; BALIAN, SIMONE C.; MORENO, ANDREA M.. Main bacterial species causing clinical disease in ornamental freshwater fish in Brazil. Folia Microbiologica, v. 66, n. 2, NOV 2020. (16/25745-7)

POOR, ANDRE P.; MORENO, LUISA Z.; MONTEIRO, MATHEUS S.; MATAJIRA, CARLOS E. C.; DUTRA, MAURICIO C.; LEAL, DIEGO F.; SILVA, ANA PAULA S.; GOMES, VASCO T. M.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; et al. Vaginal microbiota signatures in healthy and purulent vulvar discharge sows. SCIENTIFIC REPORTS, v. 12, n. 1, p. 12-pg., 2022-06-01. (19/17683-0, 16/25745-7, 19/01192-7, 15/26159-1)

MORENO, LUISA ZANOLLI; MIRAGLIA, FABIANA; DE OLIVEIRA, CAROLINA HELENA; VASCONCELLOS, SILVIO ARRUDA; HEINEMANN, MARCOS BRYAN; MORENO, ANDREA MICKE. Draft Genome Sequence of Brazilian Leptospira interrogans Serovar Pomona Strain GR5, Isolated from Apparently Healthy Gilt. GENOME ANNOUNCEMENTS, v. 6, n. 23, p. 2-pg., 2018-06-01. (13/17136-2, 16/25745-7)

MORENO, LUISA ZANOLLI; MIRAGLIA, FABIANA; DE OLIVEIRA, CAROLINA HELENA; VASCONCELLOS, SILVIO ARRUDA; HEINEMANN, MARCOS BRYAN; MORENO, ANDREA MICKE. Draft Genome Sequence of Brazilian Leptospira interrogans Serovar Pomona Strain GR5, Isolated from Apparently Healthy Gilt. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 23, JUN 2018. (13/17136-2, 16/25745-7)

DALMUTT, ANDRESSA C.; MORENO, LUISA Z.; GOMES, VASCO T. M.; CUNHA, MARCOS P. V.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; KNOBL, TEREZINHA; PEDROSO, ANTONIO CARLOS; MORENO, ANDREA M.. Characterization of bacterial contaminants of boar semen: identification by MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling. JOURNAL OF APPLIED ANIMAL RESEARCH, v. 48, n. 1, p. 559-565, JAN 1 2020. (16/25745-7, 19/18551-0)

MATAJIRA, CARLOS E. C.; MORENO, LUISA Z.; POOR, ANDRE P.; GOMES, VASCO T. M.; DALMUTT, ANDRESSA C.; PARRA, BEATRIZ M.; DE OLIVEIRA, CAROLINA H.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; CALDERARO, FRANCO F.; et al. Streptococcus suis in Brazil: Genotypic, Virulence, and Resistance Profiling of Strains Isolated from Pigs between 2001 and 2016. PATHOGENS, v. 9, n. 1, JAN 2020. (17/09515-4, 15/26159-1, 16/25745-7, 19/01192-7)

SPINDOLA, MARIA G.; CUNHA, MARCOS P. V.; MORENO, LUISA Z.; AMIGO, CRISTINA R.; SILVA, ANA P. S.; PARRA, BEATRIZ M.; POOR, ANDRE P.; DE OLIVEIRA, CAROLINA H.; PEREZ, BARBARA P.; KNOBL, TEREZINHA; et al. Genetic diversity, virulence genotype and antimicrobial resistance of uropathogenic Escherichia coli (UPEC) isolated from sows. VETERINARY QUARTERLY, v. 38, n. 1, p. 79-87, OCT 26 2018. (13/17136-2, 13/16946-0, 14/11523-7, 16/25745-7, 17/09515-4, 11/22608-5)

ABRAHAO, CARLOS R.; MORENO, LUISA Z.; SILVA, JEAN C. R.; BENITES, NILSON R.; MATAJIRA, CARLOS E. C.; FERREIRA, FERNANDO; MORENO, ANDREA M.; DIAS, RICARDO A.. Salmonella enterica in Invasive Lizard from Fernando de Noronha Archipelago: Serotyping, Antimicrobial Resistance and Molecular Epidemiology. MICROORGANISMS, v. 8, n. 12, DEC 2020. (15/26159-1, 16/25745-7)

MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; POOR, ANDRE P.; MESQUITA, RENAN E.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; AMIGO, CRISTINA R.; CHRIST, ANA PAULA G.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; et al. Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection. VETERINARY QUARTERLY, v. 38, n. 1, p. 1-8, DEC 7 2017. (15/26159-1, 16/25745-7)

GOMES, VASCO T. M.; MORENO, LUISA Z.; SILVA, ANA PAULA S.; THAKUR, SIDDHARTHA; LA RAGIONE, ROBERTO M.; MATHER, ALISON E.; MORENO, ANDREA M.. Characterization of Salmonella enterica Contamination in Pork and Poultry Meat from Sao Paulo/Brazil: Serotypes, Genotypes and Antimicrobial Resistance Profiles. PATHOGENS, v. 11, n. 3, p. 13-pg., 2022-03-01. (16/25745-7, 18/21216-5, 13/16946-0, 17/50453-2)

MONTEIRO, MATHEUS SALIBA; MATIAS, DEBORA NOVAIS; POOR, ANDRE PEGORARO; DUTRA, MAURICIO CABRAL; MORENO, LUISA ZANOLLI; PARRA, BEATRIZ MARTINS; SILVA, ANA PAULA SANTOS; CABRERA MATAJIRA, CARLOS EMILIO; DE MOURA GOMES, VASCO TULIO; FUNADA BARBOSA, MIKAELA RENATA; et al. Causes of Sow Mortality and Risks to Post-Mortem Findings in a Brazilian Intensive Swine Production System. ANIMALS, v. 12, n. 14, p. 18-pg., 2022-07-01. (19/01192-7, 19/17683-0, 15/26159-1, 16/25745-7)