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Characterization of Corynebacterium spp. strains isolated from urinary infection in pigs

Grant number: 15/26412-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2016
Effective date (End): December 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Andrea Micke Moreno
Grantee:André Pegoraro Poor
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Corynebacterium genus is found in many ecological niches, such as soil and sewage, and is usually isolated from different animal species. The zoonotic character of several toxigenic species of the genus has already been described in the literature. In pigs, although poorly studied, there are few reports of Corynebacterium species isolation, mainly on the upper respiratory tract. The aim of this study is to characterize suspicious Corynebacterium spp. strains that were isolated from urine of sows with urinary tract infection. The bacterial identification will be performed by the MALDI-TOF mass spectrometry technique and confirmed through the phylogenetic analysis of 16S rRNA gene. It will also be done the strains phenotypic characterization, with antimicrobial resistance profile determination, as well as the genotypic characterization through single enzyme amplified fragments length polymorphism (SE-AFLP). Therefore, it will be possible identify the corynebacteria species capable of causing urinary tract infection in pigs, which is a given unprecedented in Brazil. Furthermore, the antimicrobial susceptibility profile characterization of these strains will be very useful for the evaluation of the treatment protocols used in swine industry and of the resistance dissemination in the field.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
POOR, ANDRE P.; MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; PARRA, BEATRIZ M.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; DUTRA, MAURICIO C.; CHRIST, ANA PAULA G.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; MORENO, ANDREA M. Characterization of Corynebacterium diphtheriae, C. confusum and C-amycolatum isolated from sows with genitourinary infection. Veterinary Microbiology, v. 207, p. 149-152, AUG 2017. Web of Science Citations: 2.

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