Box-Behnken analysis and storage of spray-dried collagenolytic proteases from Myceliophthora thermophila submerged bioprocess

Autor(es)

Abou Hamin Neto, Youssef Ali ; Coitinho, Luciana Barbosa ; Pedro de Freitas, Luis Alexandre ; Cabral, Hamilton

• Tipo de documento: Artigo Científico
• Fonte: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY; v. 47, n. 5, p. 473-480, 2017.
• Citações Web of Science: 0

Resumo

Enzymes do not have long-term storage stability in soluble forms, thus drying methods could minimize the loss of enzymatic activity, the spray dryer removes water under high temperatures and little time. The aims of this study were to improve the stability of enzymatic extract from Myceliophthora thermophila for potential applications in industry and to evaluate the best conditions to remove the water by spray drying technique. The parameters were tested according to Box-Behnken and evaluated by analysis of variance (ANOVA), all the parameters measured were found to influence the final enzyme activity and spray drying process yield ranged from 38.65 to 63.75%. Enzyme powders showed increased storage stability than extract and maintained about 100% of collagenolytic activity after 180 days of storage at 30 degrees C. The results showed that the microbial enzymes maintained activity during the spray drying process and were stable during long-term storage; these are promising characteristics for industrial applications. (AU)

• Processo FAPESP: 11/06986-0 - Determinação da especificidade de peptidases isoladas de fungos usando peptídeos FRET como substratos
• Beneficiário: Hamilton Cabral
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/18278-2 - Bioprocesso, purificação e caracterização das peptidases produzidas pelos fungos Eupenicillium javanicum e Myceliophthora SP. e avaliação da produção de peptídeos biologicamente ativos
• Beneficiário: Youssef Ali Abou Hamin Neto
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/24703-8 - Análises proteômicas de fungos filamentosos meso e termofílicos expostos aos fatores físico e químico
• Beneficiário: Hamilton Cabral
• Linha de fomento: Auxílio à Pesquisa - Regular