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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Box-Behnken analysis and storage of spray-dried collagenolytic proteases from Myceliophthora thermophila submerged bioprocess

Full text
Author(s):
Abou Hamin Neto, Youssef Ali ; Coitinho, Luciana Barbosa ; Pedro de Freitas, Luis Alexandre ; Cabral, Hamilton
Total Authors: 4
Document type: Journal article
Source: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY; v. 47, n. 5, p. 473-480, 2017.
Web of Science Citations: 0
Abstract

Enzymes do not have long-term storage stability in soluble forms, thus drying methods could minimize the loss of enzymatic activity, the spray dryer removes water under high temperatures and little time. The aims of this study were to improve the stability of enzymatic extract from Myceliophthora thermophila for potential applications in industry and to evaluate the best conditions to remove the water by spray drying technique. The parameters were tested according to Box-Behnken and evaluated by analysis of variance (ANOVA), all the parameters measured were found to influence the final enzyme activity and spray drying process yield ranged from 38.65 to 63.75%. Enzyme powders showed increased storage stability than extract and maintained about 100% of collagenolytic activity after 180 days of storage at 30 degrees C. The results showed that the microbial enzymes maintained activity during the spray drying process and were stable during long-term storage; these are promising characteristics for industrial applications. (AU)

FAPESP's process: 11/06986-0 - Determination of the specificity of peptidases isolated from fungi using fluorescence resonance energy transfer (FRET) peptides as substrates
Grantee:Hamilton Cabral
Support Opportunities: Regular Research Grants
FAPESP's process: 12/18278-2 - Bioprocess, purification and characterization of peptidases produced by fungi Eupenicillium javanicum and Myceliophthora sp. and evaluating the production of biologically active peptides
Grantee:Youssef Ali Abou Hamin Neto
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/24703-8 - Proteomics analyses of meso and thermophilic filamentous fungi exposed to physical and chemical factors
Grantee:Hamilton Cabral
Support Opportunities: Regular Research Grants