Progesterone Decreases in vitro Indoleamine 2, 3-dioxygenase Expression in Dendritic and CD4(+) Cells from Maternal-Fetal Interface of Rats

Autor(es)

Faria da Cunha Bianchi, Pedro Kastein ; Leandro, Rafael Magdanelo ; Poscai, Aline Nayara ; Yoshinaga, Tulio ; Goncalez, Patricia Orlandini ; Kfoury Junior, Jose Roberto

• Tipo de documento: Artigo Científico
• Fonte: IMMUNOLOGICAL INVESTIGATIONS; v. 46, n. 5, p. 447-459, 2017.
• Citações Web of Science: 3

Resumo

Problem: Several mechanisms contribute to the tolerogenic state observed during pregnancy, such as the activity of the enzyme indoleamine 2, 3-dioxygenase (IDO). This initializes the catabolism of tryptophan, inducing T cells to apoptosis due to its deprivation and by the action of its catabolites in the placental microenvironment. Progesterone plays an important part on immunological tolerance mechanisms during pregnancy; however, there is no evidence it is related to IDO activity. Thus, this study aimed to investigate progesterone influence on the maternal-fetal interface of pregnant Wistar rats, by identifying IDO positive cells by immunophenotyping and flow cytometry under exogenous progesterone supplementation.Method of study: Placenta and embryo cells were cultured and separated into groups that received interferon or progesterone, supplemented or not with mifepristone. After 2 and 24 h, these were labeled with an anti-IDO and a series of antibodies specific to leucocytes and progesterone receptor and processed through flow cytometry analysis.Results: Progesterone induced a significant decrease in the expression of IDO in dendritic cells and CD4(+) lymphocytes.Conclusion: The blocking of progesterone receptor on these cells by mifepristone restored IDO expression levels and may constitute evidence of the participation of this hormone through a direct route in these cells. (AU)

• Processo FAPESP: 09/54597-2 - Hormônios de reprodução e citocinas influenciam na expressão da indoleamina 2,3-dioxigenase em cultivo celular de placenta e embrião murino e de ratas Wistar?
• Beneficiário: José Roberto Kfoury Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular