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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Progesterone Decreases in vitro Indoleamine 2, 3-dioxygenase Expression in Dendritic and CD4(+) Cells from Maternal-Fetal Interface of Rats

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Author(s):
Faria da Cunha Bianchi, Pedro Kastein ; Leandro, Rafael Magdanelo ; Poscai, Aline Nayara ; Yoshinaga, Tulio ; Goncalez, Patricia Orlandini ; Kfoury Junior, Jose Roberto
Total Authors: 6
Document type: Journal article
Source: IMMUNOLOGICAL INVESTIGATIONS; v. 46, n. 5, p. 447-459, 2017.
Web of Science Citations: 3
Abstract

Problem: Several mechanisms contribute to the tolerogenic state observed during pregnancy, such as the activity of the enzyme indoleamine 2, 3-dioxygenase (IDO). This initializes the catabolism of tryptophan, inducing T cells to apoptosis due to its deprivation and by the action of its catabolites in the placental microenvironment. Progesterone plays an important part on immunological tolerance mechanisms during pregnancy; however, there is no evidence it is related to IDO activity. Thus, this study aimed to investigate progesterone influence on the maternal-fetal interface of pregnant Wistar rats, by identifying IDO positive cells by immunophenotyping and flow cytometry under exogenous progesterone supplementation.Method of study: Placenta and embryo cells were cultured and separated into groups that received interferon or progesterone, supplemented or not with mifepristone. After 2 and 24 h, these were labeled with an anti-IDO and a series of antibodies specific to leucocytes and progesterone receptor and processed through flow cytometry analysis.Results: Progesterone induced a significant decrease in the expression of IDO in dendritic cells and CD4(+) lymphocytes.Conclusion: The blocking of progesterone receptor on these cells by mifepristone restored IDO expression levels and may constitute evidence of the participation of this hormone through a direct route in these cells. (AU)

FAPESP's process: 09/54597-2 - Does cytokines and reproductive hormones influence 2,3 indoleamine-dioxygenase expression in culture of termed placenta and fetuses cells from mice and Wistar rats?
Grantee:José Roberto Kfoury Júnior
Support type: Regular Research Grants