Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Progesterone Decreases in vitro Indoleamine 2, 3-dioxygenase Expression in Dendritic and CD4(+) Cells from Maternal-Fetal Interface of Rats

Full text
Faria da Cunha Bianchi, Pedro Kastein ; Leandro, Rafael Magdanelo ; Poscai, Aline Nayara ; Yoshinaga, Tulio ; Goncalez, Patricia Orlandini ; Kfoury Junior, Jose Roberto
Total Authors: 6
Document type: Journal article
Source: IMMUNOLOGICAL INVESTIGATIONS; v. 46, n. 5, p. 447-459, 2017.
Web of Science Citations: 3

Problem: Several mechanisms contribute to the tolerogenic state observed during pregnancy, such as the activity of the enzyme indoleamine 2, 3-dioxygenase (IDO). This initializes the catabolism of tryptophan, inducing T cells to apoptosis due to its deprivation and by the action of its catabolites in the placental microenvironment. Progesterone plays an important part on immunological tolerance mechanisms during pregnancy; however, there is no evidence it is related to IDO activity. Thus, this study aimed to investigate progesterone influence on the maternal-fetal interface of pregnant Wistar rats, by identifying IDO positive cells by immunophenotyping and flow cytometry under exogenous progesterone supplementation.Method of study: Placenta and embryo cells were cultured and separated into groups that received interferon or progesterone, supplemented or not with mifepristone. After 2 and 24 h, these were labeled with an anti-IDO and a series of antibodies specific to leucocytes and progesterone receptor and processed through flow cytometry analysis.Results: Progesterone induced a significant decrease in the expression of IDO in dendritic cells and CD4(+) lymphocytes.Conclusion: The blocking of progesterone receptor on these cells by mifepristone restored IDO expression levels and may constitute evidence of the participation of this hormone through a direct route in these cells. (AU)

FAPESP's process: 09/54597-2 - Does cytokines and reproductive hormones influence 2,3 indoleamine-dioxygenase expression in culture of termed placenta and fetuses cells from mice and Wistar rats?
Grantee:José Roberto Kfoury Júnior
Support type: Regular Research Grants